Comparison of systemic and otic administration of ofloxacin

Elena Koulich, Peter S. Roland, Karen S. Pawlowski

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To assess the feasibility of delivering of loxacin across the intact tympanic membrane; to compare middle ear bioavailability of ofloxacin after otic and systemic administrations; to determine distribution of otically delivered ofloxacin to other tissues. Study Design: A prospective, controlled animal study. Methods: Rats underwent surgery wherein the middle ear cavity was opened and filled with saline. An equivalent amount of ofloxacin was delivered intraperitoneally or into the external ear canal. Saline within the middle ear was sampled and completely replaced in 15-minute intervals for 3 hours. Blood was collected twice after the initial application of ofloxacin for high-performance liquid chromatography HPLC). Animals were sacrificed 3 hours after the initial addition of ofloxacin; the temporal bones were harvested for histological analysis; urine and colon mucosa were collected for HPLC analysis. Results: Both systemic and otic applications led to a comparable accumulation of ofloxacin in the middle ear over the 3-hour period after the initial administration. The pharmacokinetics of ofloxacin penetration into the middle ear was sporadic and subjectdependent. Both methods of administration led to drug accumulation in blood serum, urine, and colonic mucosa. Conclusions: Topical application of ofloxacin to he intact tympanic membrane allows for drug penetration into the middle ear space. Similar middle ear ofloxacin levels could be achieved with systemic and topic applications, but drug concentrations were inconsistent. The accumulation of ofloxacin in other tissues suggests applications designed to be ototopical may also result in systemic absorption.

Original languageEnglish (US)
Pages (from-to)2083-2088
Number of pages6
JournalLaryngoscope
Volume120
Issue number10
DOIs
StatePublished - Oct 2010

Fingerprint

Ofloxacin
Ear
Middle Ear
Tympanic Membrane
High Pressure Liquid Chromatography
Mucous Membrane
Urine
Pharmaceutical Preparations
Ear Canal
Temporal Bone
Biological Availability
Colon
Pharmacokinetics

Keywords

  • Antibiotics
  • Ofloxacin
  • Otitis media
  • Ototopical
  • Systemic

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Koulich, E., Roland, P. S., & Pawlowski, K. S. (2010). Comparison of systemic and otic administration of ofloxacin. Laryngoscope, 120(10), 2083-2088. https://doi.org/10.1002/lary.21088

Comparison of systemic and otic administration of ofloxacin. / Koulich, Elena; Roland, Peter S.; Pawlowski, Karen S.

In: Laryngoscope, Vol. 120, No. 10, 10.2010, p. 2083-2088.

Research output: Contribution to journalArticle

Koulich, E, Roland, PS & Pawlowski, KS 2010, 'Comparison of systemic and otic administration of ofloxacin', Laryngoscope, vol. 120, no. 10, pp. 2083-2088. https://doi.org/10.1002/lary.21088
Koulich, Elena ; Roland, Peter S. ; Pawlowski, Karen S. / Comparison of systemic and otic administration of ofloxacin. In: Laryngoscope. 2010 ; Vol. 120, No. 10. pp. 2083-2088.
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