Comparison of the antioxidant effects of Concord grape juice flavonoids and α-tocopherol on markers of oxidative stress in healthy adults

Dawn J. O'Byrne, Sridevi Devaraj, Scott M Grundy, Ishwarlal Jialal

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Background: Concord grape juice (CGJ) is a rich source of flavonoids, which have greater antioxidant efficacy in vitro than does α-tocopherol; however, the efficacies of flavonoids and α-tocopherol in vivo have not been compared. Objective: We compared the in vivo antioxidant efficacy of CGJ with that of α-tocopherol in healthy adults. Design: Subjects were randomly assigned to receive either 400 IU RRR-α-tocopherol/d (n = 17) or 10 mL CGJ · kg-1 · d-1 (n = 15) for 2 wk. Serum oxygen radical absorbance capacity, plasma protein carbonyls, urinary F2-isoprostanes, and resistance of LDL to ex vivo oxidation were measured before and after supplementation as markers of antioxidant status and oxidative stress. Results: After supplementation, plasma α-tocopherol increased 92% in subjects who received α-tocopherol (P < 0.001); plasma total and conjugated phenols increased 17% (P < 0.01) and 22% (P < 0.001), respectively, in subjects who received CGJ. There was a significant change in plasma triacylglycerols in both groups, but the concentrations were within the normal range. CGJ supplementation was associated with significantly higher triacylglycerols than was α-tocopherol supplementation. Both supplementation regimens significantly increased serum oxygen radical absorbance capacity (P < 0.001) and LDL lag time (P < 0.001) and significantly decreased the LDL oxidation rate (P < 0.01), with no significant difference in effectiveness. Protein carbonyl concentrations in native plasma decreased 20% after CGJ supplementation, which was a significantly different response than that after α-tocopherol supplementation (P < 0.05). Conclusions: In healthy adults, 10 mL CGJ · kg-1 · d-1 increased serum antioxidant capacity and protected LDL against oxidation to an extent similar to that obtained with 400 IU α-tocopherol/d but decreased native plasma protein oxidation significantly more than did α-tocopherol. CGJ flavonoids are potent antioxidants that may protect against oxidative stress and reduce the risk of free radical damage and chronic diseases.

Original languageEnglish (US)
Pages (from-to)1367-1374
Number of pages8
JournalAmerican Journal of Clinical Nutrition
Volume76
Issue number6
StatePublished - Dec 1 2002

Fingerprint

grape juice
Tocopherols
Vitis
tocopherols
Flavonoids
Oxidative Stress
oxidative stress
flavonoids
Antioxidants
antioxidants
oxidation
oxygen radical absorbance capacity
blood proteins
Blood Proteins
Triglycerides
Serum
triacylglycerols
F2-Isoprostanes
Phenols
chronic diseases

Keywords

  • α-tocopherol
  • Antioxidant
  • Flavonoids
  • Grape juice
  • LDL oxidation
  • Oxygen radical absorbance capacity
  • Protein carbonyls
  • Urinary F-isoprostanes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Comparison of the antioxidant effects of Concord grape juice flavonoids and α-tocopherol on markers of oxidative stress in healthy adults. / O'Byrne, Dawn J.; Devaraj, Sridevi; Grundy, Scott M; Jialal, Ishwarlal.

In: American Journal of Clinical Nutrition, Vol. 76, No. 6, 01.12.2002, p. 1367-1374.

Research output: Contribution to journalArticle

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abstract = "Background: Concord grape juice (CGJ) is a rich source of flavonoids, which have greater antioxidant efficacy in vitro than does α-tocopherol; however, the efficacies of flavonoids and α-tocopherol in vivo have not been compared. Objective: We compared the in vivo antioxidant efficacy of CGJ with that of α-tocopherol in healthy adults. Design: Subjects were randomly assigned to receive either 400 IU RRR-α-tocopherol/d (n = 17) or 10 mL CGJ · kg-1 · d-1 (n = 15) for 2 wk. Serum oxygen radical absorbance capacity, plasma protein carbonyls, urinary F2-isoprostanes, and resistance of LDL to ex vivo oxidation were measured before and after supplementation as markers of antioxidant status and oxidative stress. Results: After supplementation, plasma α-tocopherol increased 92{\%} in subjects who received α-tocopherol (P < 0.001); plasma total and conjugated phenols increased 17{\%} (P < 0.01) and 22{\%} (P < 0.001), respectively, in subjects who received CGJ. There was a significant change in plasma triacylglycerols in both groups, but the concentrations were within the normal range. CGJ supplementation was associated with significantly higher triacylglycerols than was α-tocopherol supplementation. Both supplementation regimens significantly increased serum oxygen radical absorbance capacity (P < 0.001) and LDL lag time (P < 0.001) and significantly decreased the LDL oxidation rate (P < 0.01), with no significant difference in effectiveness. Protein carbonyl concentrations in native plasma decreased 20{\%} after CGJ supplementation, which was a significantly different response than that after α-tocopherol supplementation (P < 0.05). Conclusions: In healthy adults, 10 mL CGJ · kg-1 · d-1 increased serum antioxidant capacity and protected LDL against oxidation to an extent similar to that obtained with 400 IU α-tocopherol/d but decreased native plasma protein oxidation significantly more than did α-tocopherol. CGJ flavonoids are potent antioxidants that may protect against oxidative stress and reduce the risk of free radical damage and chronic diseases.",
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T1 - Comparison of the antioxidant effects of Concord grape juice flavonoids and α-tocopherol on markers of oxidative stress in healthy adults

AU - O'Byrne, Dawn J.

AU - Devaraj, Sridevi

AU - Grundy, Scott M

AU - Jialal, Ishwarlal

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Background: Concord grape juice (CGJ) is a rich source of flavonoids, which have greater antioxidant efficacy in vitro than does α-tocopherol; however, the efficacies of flavonoids and α-tocopherol in vivo have not been compared. Objective: We compared the in vivo antioxidant efficacy of CGJ with that of α-tocopherol in healthy adults. Design: Subjects were randomly assigned to receive either 400 IU RRR-α-tocopherol/d (n = 17) or 10 mL CGJ · kg-1 · d-1 (n = 15) for 2 wk. Serum oxygen radical absorbance capacity, plasma protein carbonyls, urinary F2-isoprostanes, and resistance of LDL to ex vivo oxidation were measured before and after supplementation as markers of antioxidant status and oxidative stress. Results: After supplementation, plasma α-tocopherol increased 92% in subjects who received α-tocopherol (P < 0.001); plasma total and conjugated phenols increased 17% (P < 0.01) and 22% (P < 0.001), respectively, in subjects who received CGJ. There was a significant change in plasma triacylglycerols in both groups, but the concentrations were within the normal range. CGJ supplementation was associated with significantly higher triacylglycerols than was α-tocopherol supplementation. Both supplementation regimens significantly increased serum oxygen radical absorbance capacity (P < 0.001) and LDL lag time (P < 0.001) and significantly decreased the LDL oxidation rate (P < 0.01), with no significant difference in effectiveness. Protein carbonyl concentrations in native plasma decreased 20% after CGJ supplementation, which was a significantly different response than that after α-tocopherol supplementation (P < 0.05). Conclusions: In healthy adults, 10 mL CGJ · kg-1 · d-1 increased serum antioxidant capacity and protected LDL against oxidation to an extent similar to that obtained with 400 IU α-tocopherol/d but decreased native plasma protein oxidation significantly more than did α-tocopherol. CGJ flavonoids are potent antioxidants that may protect against oxidative stress and reduce the risk of free radical damage and chronic diseases.

AB - Background: Concord grape juice (CGJ) is a rich source of flavonoids, which have greater antioxidant efficacy in vitro than does α-tocopherol; however, the efficacies of flavonoids and α-tocopherol in vivo have not been compared. Objective: We compared the in vivo antioxidant efficacy of CGJ with that of α-tocopherol in healthy adults. Design: Subjects were randomly assigned to receive either 400 IU RRR-α-tocopherol/d (n = 17) or 10 mL CGJ · kg-1 · d-1 (n = 15) for 2 wk. Serum oxygen radical absorbance capacity, plasma protein carbonyls, urinary F2-isoprostanes, and resistance of LDL to ex vivo oxidation were measured before and after supplementation as markers of antioxidant status and oxidative stress. Results: After supplementation, plasma α-tocopherol increased 92% in subjects who received α-tocopherol (P < 0.001); plasma total and conjugated phenols increased 17% (P < 0.01) and 22% (P < 0.001), respectively, in subjects who received CGJ. There was a significant change in plasma triacylglycerols in both groups, but the concentrations were within the normal range. CGJ supplementation was associated with significantly higher triacylglycerols than was α-tocopherol supplementation. Both supplementation regimens significantly increased serum oxygen radical absorbance capacity (P < 0.001) and LDL lag time (P < 0.001) and significantly decreased the LDL oxidation rate (P < 0.01), with no significant difference in effectiveness. Protein carbonyl concentrations in native plasma decreased 20% after CGJ supplementation, which was a significantly different response than that after α-tocopherol supplementation (P < 0.05). Conclusions: In healthy adults, 10 mL CGJ · kg-1 · d-1 increased serum antioxidant capacity and protected LDL against oxidation to an extent similar to that obtained with 400 IU α-tocopherol/d but decreased native plasma protein oxidation significantly more than did α-tocopherol. CGJ flavonoids are potent antioxidants that may protect against oxidative stress and reduce the risk of free radical damage and chronic diseases.

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KW - Antioxidant

KW - Flavonoids

KW - Grape juice

KW - LDL oxidation

KW - Oxygen radical absorbance capacity

KW - Protein carbonyls

KW - Urinary F-isoprostanes

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JO - American Journal of Clinical Nutrition

JF - American Journal of Clinical Nutrition

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