Comparison of the distribution of magnesium in plasma determined by size exclusion chromatography and 31P NMR spectroscopy

Ana Batista, Ronald Corbett, Shirley Tidor, Ezra Castleman, Abbot Laptook, A. Dean Sherry

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The distribution of magnesium in plasma, bound to proteins (cMg), complexed to low molecular weight anions (cMg) and ionized (iMg), was compared by size exclusion chromatography and an approach using a combination of atomic absorption spectroscopy, ion selective electrodes and 31P nuclear magnetic resonance spectroscopy. The distribution of pMg: cMg: iMg was 28:13:59 as determined by chromatography and 26:14:60 as determined by the latter methodology. These results are consistent with the hypothesis that plasma proteins and weak complexing anions correspond to high and low affinity magnesium binding ligands in plasma, respectively.

Original languageEnglish (US)
Pages (from-to)3-9
Number of pages7
JournalMagnesium Research
Volume13
Issue number1
StatePublished - 2000

Keywords

  • Atomic absorption spectroscopy
  • Ion selective electrodes
  • Magnesium
  • Nuclear magnetic resonance
  • Plasma
  • Size exclusion chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry

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