Comparison of the distribution of magnesium in plasma determined by size exclusion chromatography and 31P NMR spectroscopy

Ana Batista; Ronald Corbett; Shirley Tidor; Ezra Castleman; Abbot Laptook; A. Dean Sherry

Comparison of the distribution of magnesium in plasma determined by size exclusion chromatography and ³¹P NMR spectroscopy

Ana Batista, Ronald Corbett, Shirley Tidor, Ezra Castleman, Abbot Laptook, A. Dean Sherry

Research output: Contribution to journal › Article › peer-review

Abstract

The distribution of magnesium in plasma, bound to proteins (cMg), complexed to low molecular weight anions (cMg) and ionized (iMg), was compared by size exclusion chromatography and an approach using a combination of atomic absorption spectroscopy, ion selective electrodes and ³¹P nuclear magnetic resonance spectroscopy. The distribution of pMg: cMg: iMg was 28:13:59 as determined by chromatography and 26:14:60 as determined by the latter methodology. These results are consistent with the hypothesis that plasma proteins and weak complexing anions correspond to high and low affinity magnesium binding ligands in plasma, respectively.

Original language	English (US)
Pages (from-to)	3-9
Number of pages	7
Journal	Magnesium Research
Volume	13
Issue number	1
State	Published - 2000

Keywords

Atomic absorption spectroscopy
Ion selective electrodes
Magnesium
Nuclear magnetic resonance
Plasma
Size exclusion chromatography

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Clinical Biochemistry

Cite this

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title = "Comparison of the distribution of magnesium in plasma determined by size exclusion chromatography and 31P NMR spectroscopy",

abstract = "The distribution of magnesium in plasma, bound to proteins (cMg), complexed to low molecular weight anions (cMg) and ionized (iMg), was compared by size exclusion chromatography and an approach using a combination of atomic absorption spectroscopy, ion selective electrodes and 31P nuclear magnetic resonance spectroscopy. The distribution of pMg: cMg: iMg was 28:13:59 as determined by chromatography and 26:14:60 as determined by the latter methodology. These results are consistent with the hypothesis that plasma proteins and weak complexing anions correspond to high and low affinity magnesium binding ligands in plasma, respectively.",

keywords = "Atomic absorption spectroscopy, Ion selective electrodes, Magnesium, Nuclear magnetic resonance, Plasma, Size exclusion chromatography",

author = "Ana Batista and Ronald Corbett and Shirley Tidor and Ezra Castleman and Abbot Laptook and {Dean Sherry}, A.",

year = "2000",

language = "English (US)",

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journal = "Magnesium Research",

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T1 - Comparison of the distribution of magnesium in plasma determined by size exclusion chromatography and 31P NMR spectroscopy

AU - Batista, Ana

AU - Corbett, Ronald

AU - Tidor, Shirley

AU - Castleman, Ezra

AU - Laptook, Abbot

AU - Dean Sherry, A.

PY - 2000

Y1 - 2000

N2 - The distribution of magnesium in plasma, bound to proteins (cMg), complexed to low molecular weight anions (cMg) and ionized (iMg), was compared by size exclusion chromatography and an approach using a combination of atomic absorption spectroscopy, ion selective electrodes and 31P nuclear magnetic resonance spectroscopy. The distribution of pMg: cMg: iMg was 28:13:59 as determined by chromatography and 26:14:60 as determined by the latter methodology. These results are consistent with the hypothesis that plasma proteins and weak complexing anions correspond to high and low affinity magnesium binding ligands in plasma, respectively.

AB - The distribution of magnesium in plasma, bound to proteins (cMg), complexed to low molecular weight anions (cMg) and ionized (iMg), was compared by size exclusion chromatography and an approach using a combination of atomic absorption spectroscopy, ion selective electrodes and 31P nuclear magnetic resonance spectroscopy. The distribution of pMg: cMg: iMg was 28:13:59 as determined by chromatography and 26:14:60 as determined by the latter methodology. These results are consistent with the hypothesis that plasma proteins and weak complexing anions correspond to high and low affinity magnesium binding ligands in plasma, respectively.

KW - Atomic absorption spectroscopy

KW - Ion selective electrodes

KW - Magnesium

KW - Nuclear magnetic resonance

KW - Plasma

KW - Size exclusion chromatography

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AN - SCOPUS:0034151591

SN - 0953-1424

VL - 13

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JO - Magnesium Research

JF - Magnesium Research

IS - 1

ER -

Comparison of the distribution of magnesium in plasma determined by size exclusion chromatography and ³¹P NMR spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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