Comparison of the telomeric repeat amplification protocol (TRAP) to the new TRAP-eze telomerase detection kit

Shawn E. Holt, James C. Norton, Woodring E. Wright, Jerry W. Shay

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

The ribonucleoprotein, telomerase, is believed to be responsible for the maintenance of telomere length in immortal and cancer cells. A PCR-based assay for the detection of telomerase activity (TRAP assay: telomeric repeat amplification protocol) was developed, allowing fast and efficient detection of telomerase activity when sample amounts are limiting. Of the thousands of primary human tumors examined using the TRAP assay, almost 90% have been shown to exhibit telomerase activity. Thus, for the early detection of cancer and for the rapid screening of compounds and drugs in cancer therapeutics, methods for the detection of telomerase activity are rapidly emerging. The recently developed TRAP-eze® kit from Oncor, Inc. gives increased sensitivity with decrease sample processing time, allowing improved detection of telomerase activity in a large number of samples. In the present study, we have addressed some of the technical aspects and limitations of critical importance for reproducibility, reliability, and linearity of the standard TRAP assay and the TRAP-eze® kit using cell culture and clinical materials.

Original languageEnglish (US)
Pages (from-to)237-248
Number of pages12
JournalMethods in Cell Science
Volume18
Issue number3
DOIs
StatePublished - 1996

Fingerprint

Telomerase
Amplification
Assays
Early Detection of Cancer
Telomere Homeostasis
Neoplasms
Ribonucleoproteins
Cell culture
Tumors
Screening
Cell Culture Techniques
Cells
Polymerase Chain Reaction
Processing
Pharmaceutical Preparations

Keywords

  • Cancer
  • Cellular immortality
  • Senescence
  • Telomerase
  • Telomeres

ASJC Scopus subject areas

  • Cell Biology

Cite this

Comparison of the telomeric repeat amplification protocol (TRAP) to the new TRAP-eze telomerase detection kit. / Holt, Shawn E.; Norton, James C.; Wright, Woodring E.; Shay, Jerry W.

In: Methods in Cell Science, Vol. 18, No. 3, 1996, p. 237-248.

Research output: Contribution to journalArticle

Holt, Shawn E. ; Norton, James C. ; Wright, Woodring E. ; Shay, Jerry W. / Comparison of the telomeric repeat amplification protocol (TRAP) to the new TRAP-eze telomerase detection kit. In: Methods in Cell Science. 1996 ; Vol. 18, No. 3. pp. 237-248.
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