Comparison of two aerosol-based detectors for the analysis of gabapentin in pharmaceutical formulations by hydrophilic interaction chromatography

Shaodong Jia, Jeong Hill Park, Jeongmi Lee, Sung Won Kwon

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Comparison of hydrophilic interaction chromatography (HILIC) columns coupled with an evaporative light scattering detector (ELSD) or charged aerosol detector (CAD) was done for the detection of gabapentin in pharmaceutical formulations. The chromatographic separations were achieved on four HILIC columns: ZIC HILIC, ZIC pHILIC, Luna HILIC, and Atlantis HILIC. Experimental factors such as mobile phase composition, acetonitrile content, and mobile phase pH were evaluated. Validation of method was done in terms of linearity, sensitivity, accuracy, and precision. The performance of ELSD detection method is comparable to that of CAD. The intra-day and inter-day variations were below 1.7% and 3.2% for CAD and 2.8%, and 3.4% for ELSD, respectively. In addition, detection sensitivities of ELSD, CAD, and UV detectors were also compared for HILIC and reversed phase (RP) modes and the highest sensitivities were obtained in the HILIC mode when connected with CAD and ELSD. The developed HILIC aerosol based detection methods were successfully applied to the analysis of gabapentin in commercial tablets and capsules.

Original languageEnglish (US)
Pages (from-to)2301-2306
Number of pages6
JournalTalanta
Volume85
Issue number5
DOIs
StatePublished - Oct 15 2011

Keywords

  • CAD
  • ELSD
  • Gabapentin
  • HILIC
  • RP
  • UV

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Comparison of two aerosol-based detectors for the analysis of gabapentin in pharmaceutical formulations by hydrophilic interaction chromatography'. Together they form a unique fingerprint.

Cite this