Compartmentation of glycolysis and glycogenolysis in the perfused rat heart

Nick Anousis, Rui A. Carvalho, Piyu Zhao, Craig R. Malloy, A. Dean Sherry

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Developing methods that can detect compartmentation of metabolic pathways in intact tissues may be important for understanding energy demand and supply. In this study, we investigated compartmentation of glycolysis and glycogenolysis in the isolated perfused rat heart using 13C NMR isotopomer analysis. Rat hearts previously depleted of myocardial glycogen were perfused with 5.5 mM [U-13C]glucose plus 50 mU/mL insulin until newly synthesized glycogen recovered to new steady-state levels (∼60% of pre-depleted values). After a short wash-out period, the perfusate glucose was then switched to [1-13C]glucose, and glycolysis and glycogenolysis were stimulated by addition of glucagon (1 μg/ml). A 13C NMR multiplet analysis of the methyl resonance of lactate provided an estimate of pyruvate derived from glucose vs glycogen while a multiplet analysis of the C4 resonance of glutamate provided an estimate of acetyl-CoA derived from glycolytic pyruvate vs glycogenolytic pyruvate. These two indices were not equivalent and their difference was further magnified in the presence of insulin during the stimulation phase. These combined observations are consistent with functional compartmentation of glycolytic and glycogenolytic enzymes that allows pyruvate generated by these two processes to be distinguished at the level of lactate and acetyl-CoA.

Original languageEnglish (US)
Pages (from-to)51-59
Number of pages9
JournalNMR in Biomedicine
Volume17
Issue number2
DOIs
StatePublished - Apr 2004

Fingerprint

Glycogenolysis
Glycolysis
Pyruvic Acid
Rats
Glycogen
Glucose
Acetyl Coenzyme A
Nuclear magnetic resonance
Insulin
Metabolic Networks and Pathways
Glucagon
Glutamic Acid
Lactic Acid
Tissue
Enzymes
Carbon-13 Magnetic Resonance Spectroscopy

Keywords

  • Glycogenolysis
  • Glycolysis
  • Metabolic compartmentation
  • NMR isotopomer analysis

ASJC Scopus subject areas

  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Biophysics

Cite this

Compartmentation of glycolysis and glycogenolysis in the perfused rat heart. / Anousis, Nick; Carvalho, Rui A.; Zhao, Piyu; Malloy, Craig R.; Sherry, A. Dean.

In: NMR in Biomedicine, Vol. 17, No. 2, 04.2004, p. 51-59.

Research output: Contribution to journalArticle

Anousis, Nick ; Carvalho, Rui A. ; Zhao, Piyu ; Malloy, Craig R. ; Sherry, A. Dean. / Compartmentation of glycolysis and glycogenolysis in the perfused rat heart. In: NMR in Biomedicine. 2004 ; Vol. 17, No. 2. pp. 51-59.
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