Competition between TIAM1 and Membranes Balances Endophilin A3 Activity in Cancer Metastasis

Kumud R. Poudel, Minna Roh-Johnson, Allen Su, Thuong Ho, Haritha Mathsyaraja, Sarah Anderson, William M. Grady, Cecilia B. Moens, Maralice Conacci-Sorrell, Robert N. Eisenman, Jihong Bai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Normal cells acquire aggressive behavior by modifying signaling pathways. For instance, alteration of endocytosis profoundly impacts both proliferation and migration during tumorigenesis. Here we investigate the mechanisms that enable the endocytic machinery to coordinate these processes. We show that a membrane curvature-sensing protein, endophilin A3, promotes growth and migration of colon cancer cells through two competing mechanisms: an endocytosis pathway that is required for proliferation and a GTPase regulatory pathway that controls cell motility. EndoA3 stimulates cell migration by binding the Rac GEF TIAM1 leading to activation of small GTPases. Competing interactions of EndoA3 with membrane versus TIAM1 modulate hyperproliferative and metastatic phenotypes. Disruption of EndoA3-membrane interactions stimulates TIAM1 and small GTPases in vitro, and further promotes pro-metastatic phenotypes in vivo. Together, these results uncover a coupling mechanism, by which EndoA3 promotes growth and migration of colon cancers, by linking membrane dynamics to GTPase regulation. Poudel et al. show that endophilin A3 (EndoA3) modulates proliferation and migration of colorectal cancer cells using two distinct pathways, i.e., EndoA3 facilitates cell proliferation by enhancing endocytosis, and it promotes migration by activating TIAM1 and Rac1. Modulation of the two pathways tunes tumor progression in vivo.

Original languageEnglish (US)
Pages (from-to)738-752.e6
JournalDevelopmental Cell
Volume45
Issue number6
DOIs
StatePublished - Jun 18 2018

Fingerprint

Endocytosis
Neoplasm Metastasis
Membranes
Monomeric GTP-Binding Proteins
GTP Phosphohydrolases
Colonic Neoplasms
Cell Movement
Neoplasms
Cells
Phenotype
Cell proliferation
Growth
Machinery
Tumors
Colorectal Neoplasms
Carcinogenesis
Chemical activation
Cell Proliferation
Modulation
Proteins

Keywords

  • BAR domain
  • cell migration
  • endocytosis
  • endophilin
  • membrane remodeling
  • metastasis
  • PTEN
  • Rac1
  • TIAM1

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Poudel, K. R., Roh-Johnson, M., Su, A., Ho, T., Mathsyaraja, H., Anderson, S., ... Bai, J. (2018). Competition between TIAM1 and Membranes Balances Endophilin A3 Activity in Cancer Metastasis. Developmental Cell, 45(6), 738-752.e6. https://doi.org/10.1016/j.devcel.2018.05.021

Competition between TIAM1 and Membranes Balances Endophilin A3 Activity in Cancer Metastasis. / Poudel, Kumud R.; Roh-Johnson, Minna; Su, Allen; Ho, Thuong; Mathsyaraja, Haritha; Anderson, Sarah; Grady, William M.; Moens, Cecilia B.; Conacci-Sorrell, Maralice; Eisenman, Robert N.; Bai, Jihong.

In: Developmental Cell, Vol. 45, No. 6, 18.06.2018, p. 738-752.e6.

Research output: Contribution to journalArticle

Poudel, KR, Roh-Johnson, M, Su, A, Ho, T, Mathsyaraja, H, Anderson, S, Grady, WM, Moens, CB, Conacci-Sorrell, M, Eisenman, RN & Bai, J 2018, 'Competition between TIAM1 and Membranes Balances Endophilin A3 Activity in Cancer Metastasis', Developmental Cell, vol. 45, no. 6, pp. 738-752.e6. https://doi.org/10.1016/j.devcel.2018.05.021
Poudel KR, Roh-Johnson M, Su A, Ho T, Mathsyaraja H, Anderson S et al. Competition between TIAM1 and Membranes Balances Endophilin A3 Activity in Cancer Metastasis. Developmental Cell. 2018 Jun 18;45(6):738-752.e6. https://doi.org/10.1016/j.devcel.2018.05.021
Poudel, Kumud R. ; Roh-Johnson, Minna ; Su, Allen ; Ho, Thuong ; Mathsyaraja, Haritha ; Anderson, Sarah ; Grady, William M. ; Moens, Cecilia B. ; Conacci-Sorrell, Maralice ; Eisenman, Robert N. ; Bai, Jihong. / Competition between TIAM1 and Membranes Balances Endophilin A3 Activity in Cancer Metastasis. In: Developmental Cell. 2018 ; Vol. 45, No. 6. pp. 738-752.e6.
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