Complementation between mutants impaired in inorganic phosphate (Pi) transport via the phosphate-specific transport (PST) system was studied. For that purpose the transport of Pi via the alternative Pi transport (PIT) system was bioenergetically arrested. Complementation was found between pstB and phoT mutations, whereas each of these mutations failed to complement with a phoS mutation. The data obtained confirm previous studies in which the inducibility of alkaline phosphatase was used to determine complementation and indicated a polar effect of the phoS mutation on pstB and phoT.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology