Complementation tests between mutations in the phosphatespecific transport region of Escherichia coli

Ruth Brucker, Ruth Levitz, Ezra Yagil, Ilan Friedberg

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Abstract

Complementation between mutants impaired in inorganic phosphate (Pi) transport via the phosphate-specific transport (PST) system was studied. For that purpose the transport of Pi via the alternative Pi transport (PIT) system was bioenergetically arrested. Complementation was found between pstB and phoT mutations, whereas each of these mutations failed to complement with a phoS mutation. The data obtained confirm previous studies in which the inducibility of alkaline phosphatase was used to determine complementation and indicated a polar effect of the phoS mutation on pstB and phoT.

Original languageEnglish (US)
Pages (from-to)303-307
Number of pages5
JournalCurrent Microbiology
Volume10
Issue number5
DOIs
StatePublished - Sep 1 1984

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ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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