Complete primary structure and tissue expression of chicken pectoralis M-protein

Jiro Noguchi, Masashi Yanagisawa, Michihiro Imamura, Yoshitoshi Kasuya, Takeshi Sakurai, Takeshi Tanaka, Tomoh Masaki

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

M-Protein (165 kDa) is a structural constituent of myofibrillar M-band in striated muscle. We generated a monoclonal antibody which recognized a 165-kDa protein from chicken pectoralis muscle in immunoblot analysis and stained the M-band under immunofluorescence microscopy. By screening a λgt11 cDNA library from chicken embryonic pectoralis muscle with this antibody, we isolated a cDNA clone encoding the M-protein. Northern blot analysis showed that M-protein mRNA is expressed in pectoralis and cardiac muscle but not in gizzard smooth muscle or non-muscle tissues. Moreover, the anterior latissimus dorsi muscle, which consists almost exclusively of slow fiber types, contains no detectable levels of the mRNA. The full-length cDNA sequence predicted a 1,450-ammo acid polypeptide with a calculated molecular weight of 163 × 103. The encoded protein contains several copies of two different repetitive motifs: five copies of fibronectin type III repeats are in the middle part of the predicted molecule, and two and four copies of the immunoglobulin C2-type repeats are located toward the NH2-terminal and COOH-terminal regions, respectively. This indicates that M-protein, along with other thick filament-associated proteins such as C-protein, twichin, and titin, belongs to the superfamily of cytoskeletal proteins with immunoglobulin/fibronectin repeats.

Original languageEnglish (US)
Pages (from-to)20302-20310
Number of pages9
JournalJournal of Biological Chemistry
Volume267
Issue number28
StatePublished - Oct 5 1992

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Chickens
Muscle
Tissue
Pectoralis Muscles
Proteins
Fibronectins
Immunoglobulins
Complementary DNA
Connectin
Avian Gizzard
Messenger RNA
Cytoskeletal Proteins
Striated Muscle
Superficial Back Muscles
Protein C
Gene Library
Fluorescence Microscopy
Northern Blotting
Smooth Muscle
Myocardium

ASJC Scopus subject areas

  • Biochemistry

Cite this

Noguchi, J., Yanagisawa, M., Imamura, M., Kasuya, Y., Sakurai, T., Tanaka, T., & Masaki, T. (1992). Complete primary structure and tissue expression of chicken pectoralis M-protein. Journal of Biological Chemistry, 267(28), 20302-20310.

Complete primary structure and tissue expression of chicken pectoralis M-protein. / Noguchi, Jiro; Yanagisawa, Masashi; Imamura, Michihiro; Kasuya, Yoshitoshi; Sakurai, Takeshi; Tanaka, Takeshi; Masaki, Tomoh.

In: Journal of Biological Chemistry, Vol. 267, No. 28, 05.10.1992, p. 20302-20310.

Research output: Contribution to journalArticle

Noguchi, J, Yanagisawa, M, Imamura, M, Kasuya, Y, Sakurai, T, Tanaka, T & Masaki, T 1992, 'Complete primary structure and tissue expression of chicken pectoralis M-protein', Journal of Biological Chemistry, vol. 267, no. 28, pp. 20302-20310.
Noguchi J, Yanagisawa M, Imamura M, Kasuya Y, Sakurai T, Tanaka T et al. Complete primary structure and tissue expression of chicken pectoralis M-protein. Journal of Biological Chemistry. 1992 Oct 5;267(28):20302-20310.
Noguchi, Jiro ; Yanagisawa, Masashi ; Imamura, Michihiro ; Kasuya, Yoshitoshi ; Sakurai, Takeshi ; Tanaka, Takeshi ; Masaki, Tomoh. / Complete primary structure and tissue expression of chicken pectoralis M-protein. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 28. pp. 20302-20310.
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