Complex wavefront corrections for deep tissue focusing using low coherence backscattered light

Reto Fiolka, Ke Si, Meng Cui

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Aberrations and random scattering severely limit optical imaging in deep tissue. Adaptive optics can in principle drastically extend the penetration depth and improve the image quality. However, for random scattering media a large number of spatial modes need to be measured and controlled to restore a diffraction limited focus. Here, we present a parallel wavefront optimization method using backscattered light as a feedback. Spatial confinement of the feedback signal is realized with a confocal pinhole and coherence gating. We show in simulations and experiments that this approach enables focusing deep into tissue over up to six mean scattering path lengths. Experimentally the technique was tested on tissue phantoms and fixed brain slices.

Original languageEnglish (US)
Pages (from-to)16532-16543
Number of pages12
JournalOptics Express
Volume20
Issue number15
DOIs
StatePublished - Jul 16 2012

Fingerprint

scattering
pinholes
adaptive optics
brain
aberration
penetration
optimization
diffraction
simulation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Complex wavefront corrections for deep tissue focusing using low coherence backscattered light. / Fiolka, Reto; Si, Ke; Cui, Meng.

In: Optics Express, Vol. 20, No. 15, 16.07.2012, p. 16532-16543.

Research output: Contribution to journalArticle

Fiolka, Reto ; Si, Ke ; Cui, Meng. / Complex wavefront corrections for deep tissue focusing using low coherence backscattered light. In: Optics Express. 2012 ; Vol. 20, No. 15. pp. 16532-16543.
@article{b5fc9403970b44f99b616b17c26f7685,
title = "Complex wavefront corrections for deep tissue focusing using low coherence backscattered light",
abstract = "Aberrations and random scattering severely limit optical imaging in deep tissue. Adaptive optics can in principle drastically extend the penetration depth and improve the image quality. However, for random scattering media a large number of spatial modes need to be measured and controlled to restore a diffraction limited focus. Here, we present a parallel wavefront optimization method using backscattered light as a feedback. Spatial confinement of the feedback signal is realized with a confocal pinhole and coherence gating. We show in simulations and experiments that this approach enables focusing deep into tissue over up to six mean scattering path lengths. Experimentally the technique was tested on tissue phantoms and fixed brain slices.",
author = "Reto Fiolka and Ke Si and Meng Cui",
year = "2012",
month = "7",
day = "16",
doi = "10.1364/OE.20.016532",
language = "English (US)",
volume = "20",
pages = "16532--16543",
journal = "Optics Express",
issn = "1094-4087",
publisher = "The Optical Society",
number = "15",

}

TY - JOUR

T1 - Complex wavefront corrections for deep tissue focusing using low coherence backscattered light

AU - Fiolka, Reto

AU - Si, Ke

AU - Cui, Meng

PY - 2012/7/16

Y1 - 2012/7/16

N2 - Aberrations and random scattering severely limit optical imaging in deep tissue. Adaptive optics can in principle drastically extend the penetration depth and improve the image quality. However, for random scattering media a large number of spatial modes need to be measured and controlled to restore a diffraction limited focus. Here, we present a parallel wavefront optimization method using backscattered light as a feedback. Spatial confinement of the feedback signal is realized with a confocal pinhole and coherence gating. We show in simulations and experiments that this approach enables focusing deep into tissue over up to six mean scattering path lengths. Experimentally the technique was tested on tissue phantoms and fixed brain slices.

AB - Aberrations and random scattering severely limit optical imaging in deep tissue. Adaptive optics can in principle drastically extend the penetration depth and improve the image quality. However, for random scattering media a large number of spatial modes need to be measured and controlled to restore a diffraction limited focus. Here, we present a parallel wavefront optimization method using backscattered light as a feedback. Spatial confinement of the feedback signal is realized with a confocal pinhole and coherence gating. We show in simulations and experiments that this approach enables focusing deep into tissue over up to six mean scattering path lengths. Experimentally the technique was tested on tissue phantoms and fixed brain slices.

UR - http://www.scopus.com/inward/record.url?scp=84864122226&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864122226&partnerID=8YFLogxK

U2 - 10.1364/OE.20.016532

DO - 10.1364/OE.20.016532

M3 - Article

AN - SCOPUS:84864122226

VL - 20

SP - 16532

EP - 16543

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 15

ER -