Composite skin graft

Frozen dermal allografts support the engraftment and expansion of autologous epidermis

E. L. Heck, P. R. Bergstresser, C. R. Baxter

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Rapid closure of burn wounds significantly reduces the complications associated with thermal injury. Successful wound coverage, however, is often limited by the lack of suitable autografts. To circumvent this limitation a composite graft was developed which combines the utility and availability of allogeneic skin with the permanence of an autograft. Composite grafts were first employed in a rat wound model and subsequently to treat six patients with thermal injuries. In experiments with rats, full-thickness excised (1'') wounds were prepared on thoracic walls, covered with previously frozen allograft skin, dressed, and secured. Five days later, the dead epidermis was removed and trypsin-disaggregated syngeneic epidermal cells applied to the coverage could be observed within 7 to 10 days. In eight patients, split-thickness skin bank allografts were placed on full-thickness burn wounds. Four days later the dead epidermis was removed and vacuum blister-prepared sheets of autologous epidermis grafted to the exposed dermal surface. In all eight patients successful engraftment ensued. Increased pigmentation at the site of each original epidermal graft confirmed the stability of underlying allograft dermis. Epidermal expansion ranged from 1:20 to 1:100. All patients were followed from 10 to 12 months with no demonstrated graft loss or significant wound contracture. Composite skin grafts which combine allogeneic dermis and an expanded autologous epidermis can effect rapid wound closure and will remain stable without evidence of rejection or graft breakdown for at least 12 months.

Original languageEnglish (US)
Pages (from-to)106-112
Number of pages7
JournalJournal of Trauma
Volume25
Issue number2
StatePublished - 1985

Fingerprint

Epidermis
Allografts
Transplants
Skin
Wounds and Injuries
Autografts
Dermis
Hot Temperature
Pigmentation
Graft Rejection
Thoracic Wall
Contracture
Blister
Vacuum
Trypsin

ASJC Scopus subject areas

  • Surgery

Cite this

Composite skin graft : Frozen dermal allografts support the engraftment and expansion of autologous epidermis. / Heck, E. L.; Bergstresser, P. R.; Baxter, C. R.

In: Journal of Trauma, Vol. 25, No. 2, 1985, p. 106-112.

Research output: Contribution to journalArticle

Heck, E. L. ; Bergstresser, P. R. ; Baxter, C. R. / Composite skin graft : Frozen dermal allografts support the engraftment and expansion of autologous epidermis. In: Journal of Trauma. 1985 ; Vol. 25, No. 2. pp. 106-112.
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