Abstract
Proton NMR spectroscopy at 7 Tesla (7T) was evaluated as a new method to quantify human fat composition noninvasively. In validation experiments, the composition of a known mixture of triolein, tristearin, and trilinolein agreed well with measurements by 1H NMR spectroscopy. Triglycerides in calf subcutaneous tissue and tibial bone marrow were examined in 20 healthy subjects by 1H spectroscopy. Ten well-resolved proton resonances from triglycerides were detected using stimulated echo acquisition mode sequence and small voxel (~0.1 ml), and T1 and T2 were measured. Triglyceride composition was not different between calf subcutaneous adipose tissue and tibial marrow for a given subject, and its variation among subjects, as a result of diet and genetic differences, fell in a narrow range. After correction for differential relaxation effects, the marrow fat composition was 29.1 ± 3.5% saturated, 46.4 ± 4.8% monounsaturated, and 24.5 ± 3.1% diunsaturated, compared with adipose fat composition, 27.1 ± 4.2% saturated, 49.6 ± 5.7% monounsaturated, and 23.4 ± 3.9% diunsaturated. Proton spectroscopy at 7T offers a simple, fast, noninvasive, and painless method for obtaining detailed information about lipid composition in humans, and the sensitivity and resolution of the method may facilitate longitudinal monitoring of changes in lipid composition in response to diet, exercise, and disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2055-2062 |
| Number of pages | 8 |
| Journal | Journal of Lipid Research |
| Volume | 49 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2008 |
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Keywords
- Bone marrow
- Fatty acids
- In vivo
- Lipid composition
- Metabolism
- Musculoskeletal
- Spectroscopy
- Subcutaneous fat
- Triglycerides
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Endocrinology
Cite this
Composition of adipose tissue and marrow fat in humans by 1H NMR at 7 Tesla. / Ren, Jimin; Dimitrov, Ivan; Sherry, A. Dean; Malloy, Craig R.
In: Journal of Lipid Research, Vol. 49, No. 9, 09.2008, p. 2055-2062.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Composition of adipose tissue and marrow fat in humans by 1H NMR at 7 Tesla
AU - Ren, Jimin
AU - Dimitrov, Ivan
AU - Sherry, A. Dean
AU - Malloy, Craig R.
PY - 2008/9
Y1 - 2008/9
N2 - Proton NMR spectroscopy at 7 Tesla (7T) was evaluated as a new method to quantify human fat composition noninvasively. In validation experiments, the composition of a known mixture of triolein, tristearin, and trilinolein agreed well with measurements by 1H NMR spectroscopy. Triglycerides in calf subcutaneous tissue and tibial bone marrow were examined in 20 healthy subjects by 1H spectroscopy. Ten well-resolved proton resonances from triglycerides were detected using stimulated echo acquisition mode sequence and small voxel (~0.1 ml), and T1 and T2 were measured. Triglyceride composition was not different between calf subcutaneous adipose tissue and tibial marrow for a given subject, and its variation among subjects, as a result of diet and genetic differences, fell in a narrow range. After correction for differential relaxation effects, the marrow fat composition was 29.1 ± 3.5% saturated, 46.4 ± 4.8% monounsaturated, and 24.5 ± 3.1% diunsaturated, compared with adipose fat composition, 27.1 ± 4.2% saturated, 49.6 ± 5.7% monounsaturated, and 23.4 ± 3.9% diunsaturated. Proton spectroscopy at 7T offers a simple, fast, noninvasive, and painless method for obtaining detailed information about lipid composition in humans, and the sensitivity and resolution of the method may facilitate longitudinal monitoring of changes in lipid composition in response to diet, exercise, and disease.
AB - Proton NMR spectroscopy at 7 Tesla (7T) was evaluated as a new method to quantify human fat composition noninvasively. In validation experiments, the composition of a known mixture of triolein, tristearin, and trilinolein agreed well with measurements by 1H NMR spectroscopy. Triglycerides in calf subcutaneous tissue and tibial bone marrow were examined in 20 healthy subjects by 1H spectroscopy. Ten well-resolved proton resonances from triglycerides were detected using stimulated echo acquisition mode sequence and small voxel (~0.1 ml), and T1 and T2 were measured. Triglyceride composition was not different between calf subcutaneous adipose tissue and tibial marrow for a given subject, and its variation among subjects, as a result of diet and genetic differences, fell in a narrow range. After correction for differential relaxation effects, the marrow fat composition was 29.1 ± 3.5% saturated, 46.4 ± 4.8% monounsaturated, and 24.5 ± 3.1% diunsaturated, compared with adipose fat composition, 27.1 ± 4.2% saturated, 49.6 ± 5.7% monounsaturated, and 23.4 ± 3.9% diunsaturated. Proton spectroscopy at 7T offers a simple, fast, noninvasive, and painless method for obtaining detailed information about lipid composition in humans, and the sensitivity and resolution of the method may facilitate longitudinal monitoring of changes in lipid composition in response to diet, exercise, and disease.
KW - Bone marrow
KW - Fatty acids
KW - In vivo
KW - Lipid composition
KW - Metabolism
KW - Musculoskeletal
KW - Spectroscopy
KW - Subcutaneous fat
KW - Triglycerides
UR - http://www.scopus.com/inward/record.url?scp=53149105563&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=53149105563&partnerID=8YFLogxK
U2 - 10.1194/jlr.D800010-JLR200
DO - 10.1194/jlr.D800010-JLR200
M3 - Article
VL - 49
SP - 2055
EP - 2062
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 9
ER -