Composition of adipose tissue and marrow fat in humans by 1H NMR at 7 Tesla

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

Proton NMR spectroscopy at 7 Tesla (7T) was evaluated as a new method to quantify human fat composition noninvasively. In validation experiments, the composition of a known mixture of triolein, tristearin, and trilinolein agreed well with measurements by 1H NMR spectroscopy. Triglycerides in calf subcutaneous tissue and tibial bone marrow were examined in 20 healthy subjects by 1H spectroscopy. Ten well-resolved proton resonances from triglycerides were detected using stimulated echo acquisition mode sequence and small voxel (~0.1 ml), and T1 and T2 were measured. Triglyceride composition was not different between calf subcutaneous adipose tissue and tibial marrow for a given subject, and its variation among subjects, as a result of diet and genetic differences, fell in a narrow range. After correction for differential relaxation effects, the marrow fat composition was 29.1 ± 3.5% saturated, 46.4 ± 4.8% monounsaturated, and 24.5 ± 3.1% diunsaturated, compared with adipose fat composition, 27.1 ± 4.2% saturated, 49.6 ± 5.7% monounsaturated, and 23.4 ± 3.9% diunsaturated. Proton spectroscopy at 7T offers a simple, fast, noninvasive, and painless method for obtaining detailed information about lipid composition in humans, and the sensitivity and resolution of the method may facilitate longitudinal monitoring of changes in lipid composition in response to diet, exercise, and disease.

Original languageEnglish (US)
Pages (from-to)2055-2062
Number of pages8
JournalJournal of Lipid Research
Volume49
Issue number9
DOIs
StatePublished - Sep 2008

Fingerprint

Protons
Adipose Tissue
Triglycerides
Bone Marrow
Fats
Nuclear magnetic resonance
Tissue
Spectrum Analysis
Magnetic Resonance Spectroscopy
Chemical analysis
Triolein
Diet
Lipids
Subcutaneous Fat
Subcutaneous Tissue
Nutrition
Healthy Volunteers
Nuclear magnetic resonance spectroscopy
Exercise
Spectroscopy

Keywords

  • Bone marrow
  • Fatty acids
  • In vivo
  • Lipid composition
  • Metabolism
  • Musculoskeletal
  • Spectroscopy
  • Subcutaneous fat
  • Triglycerides

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Composition of adipose tissue and marrow fat in humans by 1H NMR at 7 Tesla. / Ren, Jimin; Dimitrov, Ivan; Sherry, A. Dean; Malloy, Craig R.

In: Journal of Lipid Research, Vol. 49, No. 9, 09.2008, p. 2055-2062.

Research output: Contribution to journalArticle

@article{627a7b366b8a46b88cca210c0ea8818c,
title = "Composition of adipose tissue and marrow fat in humans by 1H NMR at 7 Tesla",
abstract = "Proton NMR spectroscopy at 7 Tesla (7T) was evaluated as a new method to quantify human fat composition noninvasively. In validation experiments, the composition of a known mixture of triolein, tristearin, and trilinolein agreed well with measurements by 1H NMR spectroscopy. Triglycerides in calf subcutaneous tissue and tibial bone marrow were examined in 20 healthy subjects by 1H spectroscopy. Ten well-resolved proton resonances from triglycerides were detected using stimulated echo acquisition mode sequence and small voxel (~0.1 ml), and T1 and T2 were measured. Triglyceride composition was not different between calf subcutaneous adipose tissue and tibial marrow for a given subject, and its variation among subjects, as a result of diet and genetic differences, fell in a narrow range. After correction for differential relaxation effects, the marrow fat composition was 29.1 ± 3.5{\%} saturated, 46.4 ± 4.8{\%} monounsaturated, and 24.5 ± 3.1{\%} diunsaturated, compared with adipose fat composition, 27.1 ± 4.2{\%} saturated, 49.6 ± 5.7{\%} monounsaturated, and 23.4 ± 3.9{\%} diunsaturated. Proton spectroscopy at 7T offers a simple, fast, noninvasive, and painless method for obtaining detailed information about lipid composition in humans, and the sensitivity and resolution of the method may facilitate longitudinal monitoring of changes in lipid composition in response to diet, exercise, and disease.",
keywords = "Bone marrow, Fatty acids, In vivo, Lipid composition, Metabolism, Musculoskeletal, Spectroscopy, Subcutaneous fat, Triglycerides",
author = "Jimin Ren and Ivan Dimitrov and Sherry, {A. Dean} and Malloy, {Craig R.}",
year = "2008",
month = "9",
doi = "10.1194/jlr.D800010-JLR200",
language = "English (US)",
volume = "49",
pages = "2055--2062",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "9",

}

TY - JOUR

T1 - Composition of adipose tissue and marrow fat in humans by 1H NMR at 7 Tesla

AU - Ren, Jimin

AU - Dimitrov, Ivan

AU - Sherry, A. Dean

AU - Malloy, Craig R.

PY - 2008/9

Y1 - 2008/9

N2 - Proton NMR spectroscopy at 7 Tesla (7T) was evaluated as a new method to quantify human fat composition noninvasively. In validation experiments, the composition of a known mixture of triolein, tristearin, and trilinolein agreed well with measurements by 1H NMR spectroscopy. Triglycerides in calf subcutaneous tissue and tibial bone marrow were examined in 20 healthy subjects by 1H spectroscopy. Ten well-resolved proton resonances from triglycerides were detected using stimulated echo acquisition mode sequence and small voxel (~0.1 ml), and T1 and T2 were measured. Triglyceride composition was not different between calf subcutaneous adipose tissue and tibial marrow for a given subject, and its variation among subjects, as a result of diet and genetic differences, fell in a narrow range. After correction for differential relaxation effects, the marrow fat composition was 29.1 ± 3.5% saturated, 46.4 ± 4.8% monounsaturated, and 24.5 ± 3.1% diunsaturated, compared with adipose fat composition, 27.1 ± 4.2% saturated, 49.6 ± 5.7% monounsaturated, and 23.4 ± 3.9% diunsaturated. Proton spectroscopy at 7T offers a simple, fast, noninvasive, and painless method for obtaining detailed information about lipid composition in humans, and the sensitivity and resolution of the method may facilitate longitudinal monitoring of changes in lipid composition in response to diet, exercise, and disease.

AB - Proton NMR spectroscopy at 7 Tesla (7T) was evaluated as a new method to quantify human fat composition noninvasively. In validation experiments, the composition of a known mixture of triolein, tristearin, and trilinolein agreed well with measurements by 1H NMR spectroscopy. Triglycerides in calf subcutaneous tissue and tibial bone marrow were examined in 20 healthy subjects by 1H spectroscopy. Ten well-resolved proton resonances from triglycerides were detected using stimulated echo acquisition mode sequence and small voxel (~0.1 ml), and T1 and T2 were measured. Triglyceride composition was not different between calf subcutaneous adipose tissue and tibial marrow for a given subject, and its variation among subjects, as a result of diet and genetic differences, fell in a narrow range. After correction for differential relaxation effects, the marrow fat composition was 29.1 ± 3.5% saturated, 46.4 ± 4.8% monounsaturated, and 24.5 ± 3.1% diunsaturated, compared with adipose fat composition, 27.1 ± 4.2% saturated, 49.6 ± 5.7% monounsaturated, and 23.4 ± 3.9% diunsaturated. Proton spectroscopy at 7T offers a simple, fast, noninvasive, and painless method for obtaining detailed information about lipid composition in humans, and the sensitivity and resolution of the method may facilitate longitudinal monitoring of changes in lipid composition in response to diet, exercise, and disease.

KW - Bone marrow

KW - Fatty acids

KW - In vivo

KW - Lipid composition

KW - Metabolism

KW - Musculoskeletal

KW - Spectroscopy

KW - Subcutaneous fat

KW - Triglycerides

UR - http://www.scopus.com/inward/record.url?scp=53149105563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53149105563&partnerID=8YFLogxK

U2 - 10.1194/jlr.D800010-JLR200

DO - 10.1194/jlr.D800010-JLR200

M3 - Article

VL - 49

SP - 2055

EP - 2062

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 9

ER -