Compositional Control of Phase-Separated Cellular Bodies

Salman F. Banani, Allyson M. Rice, William B. Peeples, Yuan Lin, Saumya Jain, Roy Parker, Michael K. Rosen

Research output: Contribution to journalArticle

244 Scopus citations

Abstract

Cellular bodies such as P bodies and PML nuclear bodies (PML NBs) appear to be phase-separated liquids organized by multivalent interactions among proteins and RNA molecules. Although many components of various cellular bodies are known, general principles that define body composition are lacking. We modeled cellular bodies using several engineered multivalent proteins and RNA. In vitro and in cells, these scaffold molecules form phase-separated liquids that concentrate low valency client proteins. Clients partition differently depending on the ratio of scaffolds, with a sharp switch across the phase diagram diagonal. Composition can switch rapidly through changes in scaffold concentration or valency. Natural PML NBs and P bodies show analogous partitioning behavior, suggesting how their compositions could be controlled by levels of PML SUMOylation or cellular mRNA concentration, respectively. The data suggest a conceptual framework for considering the composition and control thereof of cellular bodies assembled through heterotypic multivalent interactions.

Original languageEnglish (US)
Pages (from-to)651-663
Number of pages13
JournalCell
Volume166
Issue number3
DOIs
StatePublished - Jul 28 2016

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Compositional Control of Phase-Separated Cellular Bodies'. Together they form a unique fingerprint.

  • Cite this

    Banani, S. F., Rice, A. M., Peeples, W. B., Lin, Y., Jain, S., Parker, R., & Rosen, M. K. (2016). Compositional Control of Phase-Separated Cellular Bodies. Cell, 166(3), 651-663. https://doi.org/10.1016/j.cell.2016.06.010