Computational Processing and Analysis of Dynamic Fluorescence Image Data

Jonas F. Dorn, Gaudenz Danuser, Ge Yang

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

With the many modes of live cell fluorescence imaging made possible by the rapid advances of fluorescent protein technology, researchers begin to face a new challenge: How to transform the vast amounts of unstructured image data into quantitative information for the discovery of new cell behaviors and the rigorous testing of mechanistic hypotheses? Although manual and semiautomatic computer-assisted image analysis are still used extensively, the demand for more reproducible and complete image measurements of complex cellular dynamics increases the need for fully automatic computational image processing approaches for both mechanistic studies and screening applications in cell biology. This chapter provides an overview of the issues that arise with the use of computational algorithms in live cell imaging studies, with particular emphasis on the close coordination of sample preparation, image acquisition, and computational image analysis. It also aims to introduce the terminology and central concepts of computer vision to facilitate the communication between cell biologists and computer scientists in collaborative imaging projects.

Original languageEnglish (US)
Pages (from-to)497-538
Number of pages42
JournalMethods in Cell Biology
Volume85
DOIs
StatePublished - 2008

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Fluorescence
Computer-Assisted Image Processing
Optical Imaging
Terminology
Cell Communication
Cell Biology
Research Personnel
Technology
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Computational Processing and Analysis of Dynamic Fluorescence Image Data. / Dorn, Jonas F.; Danuser, Gaudenz; Yang, Ge.

In: Methods in Cell Biology, Vol. 85, 2008, p. 497-538.

Research output: Contribution to journalArticle

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