Computational staining of pathology images to study the tumor microenvironment in lung cancer

Shidan Wang, Ruichen Rong, Donghan M. Yang, Junya Fujimoto, Shirley Yan, Ling Cai, Lin Yang, Danni Luo, Carmen Behrens, Edwin R. Parra, Bo Yao, Lin Xu, Tao Wang, Xiaowei Zhan, Ignacio I. Wistuba, John Minna, Yang Xie, Guanghua Xiao

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The spatial organization of different types of cells in tumor tissues reveals important information about the tumor microenvironment (TME). To facilitate the study of cellular spatial organization and interactions, we developed Histology-based Digital-Staining, a deep learning-based computation model, to segment the nuclei of tumor, stroma, lymphocyte, macrophage, karyorrhexis, and red blood cells from standard hematoxylin and eosin–stained pathology images in lung adenocarcinoma. Using this tool, we identified and classified cell nuclei and extracted 48 cell spatial organization-related features that characterize the TME. Using these features, we developed a prognostic model from the National Lung Screening Trial dataset, and independently validated the model in The Cancer Genome Atlas lung adenocarcinoma dataset, in which the predicted high-risk group showed significantly worse survival than the low-risk group (P = 0.001), with a HR of 2.23 (1.37–3.65) after adjusting for clinical variables. Furthermore, the image-derived TME features significantly correlated with the gene expression of biological pathways. For example, transcriptional activation of both the T-cell receptor and programmed cell death protein 1 pathways positively correlated with the density of detected lymphocytes in tumor tissues, while expression of the extracellular matrix organization pathway positively correlated with the density of stromal cells. In summary, we demonstrate that the spatial organization of different cell types is predictive of patient survival and associated with the gene expression of biological pathways.

Original languageEnglish (US)
Pages (from-to)2056-2066
Number of pages11
JournalCancer research
Volume80
Issue number10
DOIs
StatePublished - May 2020

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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