Concanavalin A increases the strength of baby hamster kidney cell attachment to substratum

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Abstract

The strength of attachment of normal and transformed baby hamster kidney cells was markedly increased when attached cells were treated with concanavalin A (Con A). The cells became less sensitive to detachment by physical shear or by treatment with trypsin or EDTA; however, their morphology, as observed by phase contrast microscopy, did not change. The effects of Con A were prevented by the simultaneous addition of either D glucose or α methyl D glucoside with the Con A. Also addition of these reagents to the attached cells after Con A treatment partially reversed the effects caused by Con A. Pretreatment of the culture flasks with Con A before cell attachment resulted in an increase in the strength of cell attachment to the culture flasks as compared to untreated controls.

Original languageEnglish (US)
Pages (from-to)602-607
Number of pages6
JournalJournal of Cell Biology
Volume58
Issue number3
StatePublished - 1973

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Concanavalin A
Cricetinae
Kidney
Phase-Contrast Microscopy
Edetic Acid
Trypsin
Glucose

ASJC Scopus subject areas

  • Cell Biology

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Concanavalin A increases the strength of baby hamster kidney cell attachment to substratum. / Grinnell, F.

In: Journal of Cell Biology, Vol. 58, No. 3, 1973, p. 602-607.

Research output: Contribution to journalArticle

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