Concerted action of RAS and G proteins in the sexual response pathways of Schizosaccharomyces pombe

Hao Peng Xu, Michael White, Stevan Marcus, Michael Wigler

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We have shown that the expression of mam2, the gene encoding the Schizosaccharomyces pombe P-factor pheromone receptor, is dependent upon components of the pheromone signal transduction pathway, including Ras1, Gpa1, Byr1 and Byr2, each of which is required for both conjugation and sporulation. Studies of the expression of mam2 in mutant S. pombe cells confirm previous conclusions, based on the ability of cells to sporulate, that the Byr1 protein kinase acts downstream of the Byr2 protein kinase and that both act downstream of Ras1, the S. pombe RAS homolog, and Gpa1, the Gα component that mediates the occupancy of the mam2 receptor. In addition, our present studies show that Ras1 and Gpa1 each act downstream from the other and hence act in concert. The Spk1 kinase, which is required for conjugation and sporulation and which is a structural and functional homolog of the vertebrate MAP kinases, is not required for mam2 expression.

Original languageEnglish (US)
Pages (from-to)50-58
Number of pages9
JournalMolecular and Cellular Biology
Volume14
Issue number1
StatePublished - 1994

Fingerprint

Schizosaccharomyces
GTP-Binding Proteins
Phosphotransferases
Pheromone Receptors
Pheromones
Protein Kinases
Vertebrates
Signal Transduction
Gene Expression

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Concerted action of RAS and G proteins in the sexual response pathways of Schizosaccharomyces pombe. / Xu, Hao Peng; White, Michael; Marcus, Stevan; Wigler, Michael.

In: Molecular and Cellular Biology, Vol. 14, No. 1, 1994, p. 50-58.

Research output: Contribution to journalArticle

@article{6e8dafbfcc0249ffb9b44a4fdc2d41f0,
title = "Concerted action of RAS and G proteins in the sexual response pathways of Schizosaccharomyces pombe",
abstract = "We have shown that the expression of mam2, the gene encoding the Schizosaccharomyces pombe P-factor pheromone receptor, is dependent upon components of the pheromone signal transduction pathway, including Ras1, Gpa1, Byr1 and Byr2, each of which is required for both conjugation and sporulation. Studies of the expression of mam2 in mutant S. pombe cells confirm previous conclusions, based on the ability of cells to sporulate, that the Byr1 protein kinase acts downstream of the Byr2 protein kinase and that both act downstream of Ras1, the S. pombe RAS homolog, and Gpa1, the Gα component that mediates the occupancy of the mam2 receptor. In addition, our present studies show that Ras1 and Gpa1 each act downstream from the other and hence act in concert. The Spk1 kinase, which is required for conjugation and sporulation and which is a structural and functional homolog of the vertebrate MAP kinases, is not required for mam2 expression.",
author = "Xu, {Hao Peng} and Michael White and Stevan Marcus and Michael Wigler",
year = "1994",
language = "English (US)",
volume = "14",
pages = "50--58",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Concerted action of RAS and G proteins in the sexual response pathways of Schizosaccharomyces pombe

AU - Xu, Hao Peng

AU - White, Michael

AU - Marcus, Stevan

AU - Wigler, Michael

PY - 1994

Y1 - 1994

N2 - We have shown that the expression of mam2, the gene encoding the Schizosaccharomyces pombe P-factor pheromone receptor, is dependent upon components of the pheromone signal transduction pathway, including Ras1, Gpa1, Byr1 and Byr2, each of which is required for both conjugation and sporulation. Studies of the expression of mam2 in mutant S. pombe cells confirm previous conclusions, based on the ability of cells to sporulate, that the Byr1 protein kinase acts downstream of the Byr2 protein kinase and that both act downstream of Ras1, the S. pombe RAS homolog, and Gpa1, the Gα component that mediates the occupancy of the mam2 receptor. In addition, our present studies show that Ras1 and Gpa1 each act downstream from the other and hence act in concert. The Spk1 kinase, which is required for conjugation and sporulation and which is a structural and functional homolog of the vertebrate MAP kinases, is not required for mam2 expression.

AB - We have shown that the expression of mam2, the gene encoding the Schizosaccharomyces pombe P-factor pheromone receptor, is dependent upon components of the pheromone signal transduction pathway, including Ras1, Gpa1, Byr1 and Byr2, each of which is required for both conjugation and sporulation. Studies of the expression of mam2 in mutant S. pombe cells confirm previous conclusions, based on the ability of cells to sporulate, that the Byr1 protein kinase acts downstream of the Byr2 protein kinase and that both act downstream of Ras1, the S. pombe RAS homolog, and Gpa1, the Gα component that mediates the occupancy of the mam2 receptor. In addition, our present studies show that Ras1 and Gpa1 each act downstream from the other and hence act in concert. The Spk1 kinase, which is required for conjugation and sporulation and which is a structural and functional homolog of the vertebrate MAP kinases, is not required for mam2 expression.

UR - http://www.scopus.com/inward/record.url?scp=0027953513&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027953513&partnerID=8YFLogxK

M3 - Article

VL - 14

SP - 50

EP - 58

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 1

ER -