Concomitant expression of receptor subtype and isopeptide of endothelin by human adrenal gland

Taihei Imai, Yukio Hirata, Satoru Eguchi, Kazuo Kanno, Kazuki Ohta, Toshiaki Emori, Aiji Sakamoto, Masashi Yanagisawa, Tomoh Masaki, Fumiaki Marumo

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

We studied whether specific receptors for endothelin (ET) isopeptide exist in human aldosterone-producing adenoma and normal adrenal cortex, and whether ET isopeptides are produced by human adrenal gland. Competitive binding studies using [125I]ET-1 as a radioligand revealed the presence of a single class of high-affinity binding sites for ET-1 with the apparent Kd of 70 ± 31 pM and Bmax of 226 ± 139 fmol/mg protein in adenoma membranes almost comparable to those in adjacent normal cortex. The apparent Ki for ET-2 and ET-3 were 89 ± 33 pM and 82 ± 16 pM, respectively. Northern blot analysis of poly(A)+ RNA of adenoma and adjacent normal cortex using cDNAs for ET receptor subtype (ETa, ETb) and ET isopeptide (ET-1, ET-3) as probes revealed that ETa and ETb receptors as well as ET isopeptides (prepro-ET-1, preproET-3) are concomitantly expressed in both tissues. Our data demonstrate for the first time that ET receptor subtype (ETa and ETb) and ET isopeptide (ET-1 and ET-3) are concomitantly expressed by human adrenal cortex, suggesting the potential role of ETs as a local mediator in human adrenal gland.

Original languageEnglish (US)
Pages (from-to)1115-1121
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number3
DOIs
StatePublished - Feb 14 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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