Conditional glycosylate in eukaryotic cells using a biocompatible chemical inducer of dimerization

Jennifer L. Czlapinski, Michael W. Schelle, Lawrence W. Miller, Scott T. Laughlin, Jennifer J. Kohler, Virginia W. Cornish, Carolyn R. Bertozzi

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Chemical inducers of dimerization (CIDs) are cell-permeable small molecules capable of dimerizing two protein targets. The most widely used CID, the natural product rapamycin and its relatives, is immunosuppressive due to interactions with endogenous targets and thus has limited utility in vivo. Here we report a new biocompatible CID, Tmp-SLF, which dimerizes E. coli DHFR and FKBP and has no endogenous mammalian targets that would lead to unwanted in vivo side effects. We employed Tmp-SLF to modulate gene expression in a yeast three-hybrid assay. Finally, we engineered the Golgi-resident glycosyltransferase FucT7 for tunable control by Tmp-SLF in mammalian cells.

Original languageEnglish (US)
Pages (from-to)13186-13187
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number40
DOIs
StatePublished - Oct 8 2008

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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