Conditioning lesions before or after spinal cord injury recruit broad genetic mechanisms that sustain axonal regeneration: Superiority to camp-mediated effects

Armin Blesch, Paul Lu, Shingo Tsukada, Laura Taylor Alto, Kasper Roet, Giovanni Coppola, Dan Geschwind, Mark H. Tuszynski

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

Previous studies indicate that peripheral nerve conditioning lesions significantly enhance central axonal regeneration via modulation of cAMP-mediated mechanisms. To gain insight into the nature and temporal dependence of neural mechanisms underlying conditioning lesion effects on central axonal regeneration, we compared the efficacy of peripheral sciatic nerve crush lesions to cAMP elevations (in lumbar dorsal root ganglia) on central sensory axonal regeneration when administered either before or after cervical spinal cord lesions. We found significantly greater effects of conditioning lesions compared to cAMP elevations on central axonal regeneration when combined with cellular grafts at the lesion site and viral neurotrophin delivery; further, these effects persisted whether conditioning lesions were applied prior to or shortly after spinal cord injury. Indeed, conditioning lesions recruited extensively greater sets of genetic mechanisms of possible relevance to axonal regeneration compared to cAMP administration, and sustained these changes for significantly greater time periods through the post-lesion period. We conclude that cAMP-mediated mechanisms account for only a portion of the potency of conditioning lesions on central axonal regeneration, and that recruitment of broader genetic mechanisms can extend the effect and duration of cellular events that support axonal growth.

Original languageEnglish (US)
Pages (from-to)162-173
Number of pages12
JournalExperimental Neurology
Volume235
Issue number1
DOIs
StatePublished - May 2012

Keywords

  • CTB
  • Dorsal column sensory
  • Lentivirus
  • Microarray
  • Neurotrophin-3
  • Regeneration
  • Spinal cord injury

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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