Abstract

13C NMR (nuclear magnetic resonance) spectroscopy of extracts from patient tumor samples provides rich information about metabolism. However, in isocitrate dehydrogenase (IDH)-mutant gliomas, 13C labeling is obscured in oncometabolite 2-hydroxyglutaric acid (2HG) by glutamate and glutamine, prompting development of a simple method to resolve the metabolites. J-coupled multiplets in 2HG were similar to glutamate and glutamine and could be clearly resolved at pH 6. A cryogenically cooled 13C probe, but not J-resolved heteronuclear single quantum coherence spectroscopy, significantly improved detection of 2HG. These methods enable the monitoring of 13C-13C spin-spin couplings in 2HG expressing IDH-mutant gliomas.

Original languageEnglish (US)
Pages (from-to)4-6
Number of pages3
JournalAnalytical biochemistry
Volume481
DOIs
StatePublished - Jun 3 2015

Keywords

  • 2-Hydroxyglutarate
  • C NMR
  • Cryoprobe
  • IDH-mutant gliomas

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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