Abstract

<sup>13</sup>C NMR (nuclear magnetic resonance) spectroscopy of extracts from patient tumor samples provides rich information about metabolism. However, in isocitrate dehydrogenase (IDH)-mutant gliomas, <sup>13</sup>C labeling is obscured in oncometabolite 2-hydroxyglutaric acid (2HG) by glutamate and glutamine, prompting development of a simple method to resolve the metabolites. J-coupled multiplets in 2HG were similar to glutamate and glutamine and could be clearly resolved at pH 6. A cryogenically cooled <sup>13</sup>C probe, but not J-resolved heteronuclear single quantum coherence spectroscopy, significantly improved detection of 2HG. These methods enable the monitoring of <sup>13</sup>C-<sup>13</sup>C spin-spin couplings in 2HG expressing IDH-mutant gliomas.

Original languageEnglish (US)
Pages (from-to)4-6
Number of pages3
JournalAnalytical Biochemistry
Volume481
DOIs
Publication statusPublished - Jun 3 2015

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Keywords

  • 2-Hydroxyglutarate
  • <sup>13</sup>C NMR
  • Cryoprobe
  • IDH-mutant gliomas

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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