CONFORMATIONAL CHANGES IN ADSORBED ALBUMIN INDICATED BY INTRINSIC LIFETIME MEASUREMENTS.

Mark R. Rainbow, S. J. Atherton, M. J. Rodgers, R. C. Eberhart

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Previous work suggests albumin undergoes a conformational change in order to bind irreversibly to substrate. Using total internal reflectance fluorimetry (TIRF) and pulse laser excitation, we measured the fluorescence lifetimes of tryptophan and other residues of bovine serum albumin monomer (BSA) in order to examine this concept. These studies support the hypothesis that the adsorbed albumin exists in the form of a two-layer deposit, each with a possible different structure. The decrease in fluorescence lifetime of BSA when it adsorbs to quartz implies a corresponding change in quantum yield. Changes in the quantum yields of the fluorophoric residues of adsorbed BSA implies that the method of quantifying protein adsorption on surfaces by monitoring intrinsic fluorescence in TIRF cells is not reliable.

Original languageEnglish (US)
Title of host publicationTransactions of the Annual Meeting of the Society for Biomaterials in conjunction with the Interna
Place of PublicationSan Antonio, TX, USA
PublisherSoc for Biomaterials
Pages2
Number of pages1
Volume8
StatePublished - 1985

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Monomers
Fluorescence
Quantum yield
Laser excitation
Quartz
Laser pulses
Deposits
Proteins
Adsorption
Monitoring
Substrates

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Rainbow, M. R., Atherton, S. J., Rodgers, M. J., & Eberhart, R. C. (1985). CONFORMATIONAL CHANGES IN ADSORBED ALBUMIN INDICATED BY INTRINSIC LIFETIME MEASUREMENTS. In Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the Interna (Vol. 8, pp. 2). San Antonio, TX, USA: Soc for Biomaterials.

CONFORMATIONAL CHANGES IN ADSORBED ALBUMIN INDICATED BY INTRINSIC LIFETIME MEASUREMENTS. / Rainbow, Mark R.; Atherton, S. J.; Rodgers, M. J.; Eberhart, R. C.

Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the Interna. Vol. 8 San Antonio, TX, USA : Soc for Biomaterials, 1985. p. 2.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rainbow, MR, Atherton, SJ, Rodgers, MJ & Eberhart, RC 1985, CONFORMATIONAL CHANGES IN ADSORBED ALBUMIN INDICATED BY INTRINSIC LIFETIME MEASUREMENTS. in Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the Interna. vol. 8, Soc for Biomaterials, San Antonio, TX, USA, pp. 2.
Rainbow MR, Atherton SJ, Rodgers MJ, Eberhart RC. CONFORMATIONAL CHANGES IN ADSORBED ALBUMIN INDICATED BY INTRINSIC LIFETIME MEASUREMENTS. In Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the Interna. Vol. 8. San Antonio, TX, USA: Soc for Biomaterials. 1985. p. 2
Rainbow, Mark R. ; Atherton, S. J. ; Rodgers, M. J. ; Eberhart, R. C. / CONFORMATIONAL CHANGES IN ADSORBED ALBUMIN INDICATED BY INTRINSIC LIFETIME MEASUREMENTS. Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the Interna. Vol. 8 San Antonio, TX, USA : Soc for Biomaterials, 1985. pp. 2
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