Conformational changes in the Escherichia coli ATP synthase b-dimer upon binding to F1-ATPase

Tarek M. Zaida, Tassilo Hornung, Oleg A. Volkov, Andrea D. Hoffman, Susan J. Pandey, John G. Wise, Pia D. Vogel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Conformational changes within the subunit b-dimer of the E. coli ATP synthase occur upon binding to the F1 sector. ESR spectra of spin-labeled b at room temperature indicated a pivotal point in the b-structure at residue 62. Spectra of frozen b ± F1 and calculated interspin distances suggested that where contact between b 2 and F1 occurs (above about residue 80), the structure of the dimer changes minimally. Between b-residues 33 and 64 inter-subunit distances in the F1-bound b-dimer were found to be too large to accommodate tightly coiled coil packing and therefore suggest a dissociation and disengagement of the dimer upon F1-binding. Mechanistic implications of this "bubble" formation in the tether domain of ATP synthase b 2 are discussed.

Original languageEnglish (US)
Pages (from-to)551-559
Number of pages9
JournalJournal of Bioenergetics and Biomembranes
Volume40
Issue number6
DOIs
StatePublished - Dec 1 2008

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Proton-Translocating ATPases
Adenosine Triphosphate
Escherichia coli
Temperature
E coli ATP synthase b subunit

Keywords

  • ATPase
  • B-dimer
  • Coiled coil
  • Conformational changes
  • ESR
  • External stalk
  • Spin-labeling

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Zaida, T. M., Hornung, T., Volkov, O. A., Hoffman, A. D., Pandey, S. J., Wise, J. G., & Vogel, P. D. (2008). Conformational changes in the Escherichia coli ATP synthase b-dimer upon binding to F1-ATPase. Journal of Bioenergetics and Biomembranes, 40(6), 551-559. https://doi.org/10.1007/s10863-008-9189-z

Conformational changes in the Escherichia coli ATP synthase b-dimer upon binding to F1-ATPase. / Zaida, Tarek M.; Hornung, Tassilo; Volkov, Oleg A.; Hoffman, Andrea D.; Pandey, Susan J.; Wise, John G.; Vogel, Pia D.

In: Journal of Bioenergetics and Biomembranes, Vol. 40, No. 6, 01.12.2008, p. 551-559.

Research output: Contribution to journalArticle

Zaida, TM, Hornung, T, Volkov, OA, Hoffman, AD, Pandey, SJ, Wise, JG & Vogel, PD 2008, 'Conformational changes in the Escherichia coli ATP synthase b-dimer upon binding to F1-ATPase', Journal of Bioenergetics and Biomembranes, vol. 40, no. 6, pp. 551-559. https://doi.org/10.1007/s10863-008-9189-z
Zaida, Tarek M. ; Hornung, Tassilo ; Volkov, Oleg A. ; Hoffman, Andrea D. ; Pandey, Susan J. ; Wise, John G. ; Vogel, Pia D. / Conformational changes in the Escherichia coli ATP synthase b-dimer upon binding to F1-ATPase. In: Journal of Bioenergetics and Biomembranes. 2008 ; Vol. 40, No. 6. pp. 551-559.
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