Conformational diversity of wild-type tau fibrils specified by templated conformation change

Bess Frost, Julian Ollesch, Holger Wille, Marc I. Diamond

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Tauopathies are sporadic and genetic neurodegenerative diseases characterized by aggregation of the microtubule-associated protein Tau. Tau pathology occurs in over 20 phenotypically distinct neurodegenerative diseases, including Alzheimer disease and frontotemporal dementia. The molecular basis of this diversity among sporadic tauopathies is unknown, but distinct fibrillar wild-type (WT) Tau conformations could play a role. Using Fourier transform infrared spectroscopy, circular dichroism, and electron microscopy, we show that WT Tau fibrils and P301L/V337M Tau fibrils have distinct secondary structures, fragilities, and morphologies. Furthermore, P301L/V337M fibrillar seeds induce WT Tau monomer to form a novel fibrillar conformation, termed WT*, that is maintained over multiple seeding reactions. WT* has secondary structure, fragility, and morphology that are similar to P301L/V337M fibrils and distinct from WT fibrils. WT Tau is thus capable of conformational diversity that arises via templated conformation change, as has been described for amyloid β, β 2-microglobulin, and prion proteins.

Original languageEnglish (US)
Pages (from-to)3546-3551
Number of pages6
JournalJournal of Biological Chemistry
Volume284
Issue number6
DOIs
StatePublished - Feb 6 2009

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Tauopathies
Neurodegenerative Diseases
Neurodegenerative diseases
Conformations
Alzheimer Disease
Frontotemporal Dementia
Inborn Genetic Diseases
Microtubule-Associated Proteins
Fourier Transform Infrared Spectroscopy
Circular Dichroism
Amyloid
Seeds
Electron Microscopy
Pathology
Electron microscopy
Seed
Agglomeration
Monomers
Prion Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Conformational diversity of wild-type tau fibrils specified by templated conformation change. / Frost, Bess; Ollesch, Julian; Wille, Holger; Diamond, Marc I.

In: Journal of Biological Chemistry, Vol. 284, No. 6, 06.02.2009, p. 3546-3551.

Research output: Contribution to journalArticle

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