TY - JOUR
T1 - Congenital Adrenal Hyperplasia
AU - White, P. C.
AU - New, M. I.
AU - Dupont, B.
PY - 1987/6/18
Y1 - 1987/6/18
N2 - (Second of Two Parts) Molecular Genetics of 21-Hydroxylase Deficiency It has been demonstrated that the HLA-linked defect in 21-hydroxylase deficiency involves a structural gene for the corresponding cytochrome P450 (P450c21). A bovine cDNA clone was isolated that encoded part of this enzyme (i.e., “complementary DNA,” corresponding to the messenger RNA for P450c21, linked to sequences that allowed this DNA to replicate autonomously in bacteria).52 DNA samples were extracted from leukocytes obtained from normal persons and from patients with 21-hydroxylase deficiency who carried the characteristic HLA haplotype A3;Bw47:DR7. These samples were analyzed with use of Southern blot hybridization.4,53 For this analysis,…
AB - (Second of Two Parts) Molecular Genetics of 21-Hydroxylase Deficiency It has been demonstrated that the HLA-linked defect in 21-hydroxylase deficiency involves a structural gene for the corresponding cytochrome P450 (P450c21). A bovine cDNA clone was isolated that encoded part of this enzyme (i.e., “complementary DNA,” corresponding to the messenger RNA for P450c21, linked to sequences that allowed this DNA to replicate autonomously in bacteria).52 DNA samples were extracted from leukocytes obtained from normal persons and from patients with 21-hydroxylase deficiency who carried the characteristic HLA haplotype A3;Bw47:DR7. These samples were analyzed with use of Southern blot hybridization.4,53 For this analysis,…
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U2 - 10.1056/NEJM198706183162506
DO - 10.1056/NEJM198706183162506
M3 - Review article
C2 - 3295546
AN - SCOPUS:0023226909
SN - 0028-4793
VL - 316
SP - 1580
EP - 1586
JO - New England Journal of Medicine
JF - New England Journal of Medicine
IS - 25
ER -