Conjugated avidin binds to mast cell granules

M. D. Tharp, L. L. Seelig, R. E. Tigelaar, P. R. Bergstresser

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The glycoprotein, avidin, conjugated either to the enzyme horseradish peroxidase, or to the fluorochrome dyes, fluorescein or rhodamine, identifies the granules of the mast cells in both tissues and cell suspensions. In the absence of prior fixation, mast cells were not identified with conjugated avidin; however, granules released from these cells were stained with this labeled glycoprotein. The specificity of avidin for mast cells was confirmed by the absence of conjugated avidin-positive cells in the skin of mice (S1/S1(d)) deficient in mature dermal mast cells. Electron microscopic studies confirmed that avidin binds specifically to individual mast cell granules rather than to other cellular structures. Rodent and human mast cells were readily stained with avidin conjugated to horseradish peroxidase or to either of the fluorochrome dyes. The conjugated avidin staining technique is a reliable and simple method for identifying rodent and human mast cells, one that is useful as both an investigative and a clinical tool.

Original languageEnglish (US)
Pages (from-to)27-32
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume33
Issue number1
StatePublished - 1985

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Avidin
Mast Cells
Horseradish Peroxidase
Fluorescent Dyes
Rodentia
Glycoproteins
Coloring Agents
Skin
Rhodamines
Cellular Structures
Fluorescein
Suspensions
Electrons
Staining and Labeling
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Tharp, M. D., Seelig, L. L., Tigelaar, R. E., & Bergstresser, P. R. (1985). Conjugated avidin binds to mast cell granules. Journal of Histochemistry and Cytochemistry, 33(1), 27-32.

Conjugated avidin binds to mast cell granules. / Tharp, M. D.; Seelig, L. L.; Tigelaar, R. E.; Bergstresser, P. R.

In: Journal of Histochemistry and Cytochemistry, Vol. 33, No. 1, 1985, p. 27-32.

Research output: Contribution to journalArticle

Tharp, MD, Seelig, LL, Tigelaar, RE & Bergstresser, PR 1985, 'Conjugated avidin binds to mast cell granules', Journal of Histochemistry and Cytochemistry, vol. 33, no. 1, pp. 27-32.
Tharp MD, Seelig LL, Tigelaar RE, Bergstresser PR. Conjugated avidin binds to mast cell granules. Journal of Histochemistry and Cytochemistry. 1985;33(1):27-32.
Tharp, M. D. ; Seelig, L. L. ; Tigelaar, R. E. ; Bergstresser, P. R. / Conjugated avidin binds to mast cell granules. In: Journal of Histochemistry and Cytochemistry. 1985 ; Vol. 33, No. 1. pp. 27-32.
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