Conservation of function between mammalian and plant steroid 5α- reductases

J. Li, M. G. Biswas, A. Chao, D. W. Russell, J. Chory

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Arabidopsis det2 mutants are small dark-green dwarfs displaying pleiotropic defects in light-regulated development during multiple stages of the plant life cycle. The DET2 gene encodes a protein that shares ≃40% sequence identity with mammalian steroid 5α-reductases and is implicated in the synthesis of a class of plant steroids, the brassinosteroids. Here we show that the DET2 protein, when expressed in human embryonic kidney 293 cells, catalyzes the 5α-reduction of several animal steroid substrates and has similar kinetic properties to the mammalian steroid 5α-reductase enzymes. Moreover, human steroid 5α-reductases expressed in det2 mutant plants can substitute for DET2 in brassinosteroid biosynthesis. These data indicate that DET2 is an ortholog of the mammalian steroid 5α-reductases and provide further evidence that brassinosteroids play an essential role in light-regulated plant development. The structural and functional conservation between DET2 and human steroid 5α-reductases raise interesting issues concerning the evolutionary origin of the steroid hormone signaling system.

Original languageEnglish (US)
Pages (from-to)3554-3559
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number8
DOIs
StatePublished - 1997

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Phytosterols
Oxidoreductases
Steroids
Brassinosteroids
Light
Plant Development
Life Cycle Stages
Arabidopsis
Proteins
Hormones
Kidney
Enzymes
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Conservation of function between mammalian and plant steroid 5α- reductases. / Li, J.; Biswas, M. G.; Chao, A.; Russell, D. W.; Chory, J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 8, 1997, p. 3554-3559.

Research output: Contribution to journalArticle

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