Constitutive and regulated expression of the mouse Dinb (Polκ) gene encoding DNA polymerase kappa

Susana Velasco-Miguel, James A. Richardson, Valerie L. Gerlach, Wayne C. Lai, Tianshu Gao, Lonnie D. Russell, Christa L. Hladik, Charles L. White, Errol C. Friedberg

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

A recently discovered group of novel polymerases are characterized by significantly reduced fidelity of DNA synthesis in vitro. This feature is consistent with the relaxed fidelity required for the replicative bypass of various types of base damage that frequently block high fidelity replicative polymerases. The present studies demonstrate that the specialized DNA polymerase kappa (polκ) is uniquely and preferentially expressed in the adrenal cortex and testis of the mouse, as well as in a variety of other tissues. The adrenal cortex is the sole site of detectable expression of the Polκ gene in mouse embryos. This adrenal expression pattern is consistent with a requirement for polκ for the replicative bypass of DNA base damage generated during steroid biosynthesis. The expression pattern of polκ in the testis is specific for particular stages of spermatogenesis and is distinct from the expression pattern of several other low fidelity DNA polymerases that are also expressed during spermatogenesis. The mouse (but not the human) Polκ gene is primarily regulated by the p53 gene and is upregulated in response to exposure to various DNA-damaging agents in a p53-dependent manner.

Original languageEnglish (US)
Pages (from-to)91-106
Number of pages16
JournalDNA repair
Volume2
Issue number1
DOIs
StatePublished - Jan 2 2003

Keywords

  • Adrenal gland
  • DNA damage
  • DNA polymerases
  • Mutant mice
  • Oxidative damage
  • p53-Regulated gene

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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