Constitutive expression of exogenous c-myb gene causes maturation block in monocyte-macrophage differentiation

Hiromi Yanagisawa, Toshiro Nagasawa, Satomi Kuramochi, Tsukasa Abe, Yoji Ikawa, Kazuo Todokoro

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A nuclear protooncogene c-myb has been hypothesized to play an important role in hematopoiesis, but little is known about the physiological function of the c-myb gene products. To study the role of c-myb gene expression in monocyte-macrophage differentiation and proliferation, we introduced exogenous c-myb gene into murine myelomonocytic leukemia WEHI-3B(D+) cells which can be induced to differentiate into mature monocytes with granulocyte-colony stimulation factor (G-CSF) and actinomycin D. Expression of the transfected gene was found to result in elevated levels of c-myb transcripts, which were not subject to normal down-regulation by differentiation induction. This constitutive expression of c-myb gene allowed the c-myb transfectants to differentiate into promonocytes with G-CSF and actinomycin D, but blocked further maturation from promonocytes to mature monocytes. It is concluded that normal down-regulation of c-myb gene expression during monocyte-macrophage differentiation is required for the maturation of promonocytes to mature monocytes.

Original languageEnglish (US)
Pages (from-to)380-384
Number of pages5
JournalBBA - Gene Structure and Expression
Volume1088
Issue number3
DOIs
StatePublished - Mar 26 1991

Fingerprint

myb Genes
Macrophages
Monocyte-Macrophage Precursor Cells
Monocytes
Genes
Dactinomycin
Gene expression
Gene Expression
Granulocytes
Down-Regulation
Somatostatin-Secreting Cells
Hematopoiesis
Leukemia

Keywords

  • c-myb
  • Differentiation
  • Granulocyte colony stimulation factor
  • Maturation
  • Myelomonocyte

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Constitutive expression of exogenous c-myb gene causes maturation block in monocyte-macrophage differentiation. / Yanagisawa, Hiromi; Nagasawa, Toshiro; Kuramochi, Satomi; Abe, Tsukasa; Ikawa, Yoji; Todokoro, Kazuo.

In: BBA - Gene Structure and Expression, Vol. 1088, No. 3, 26.03.1991, p. 380-384.

Research output: Contribution to journalArticle

Yanagisawa, Hiromi ; Nagasawa, Toshiro ; Kuramochi, Satomi ; Abe, Tsukasa ; Ikawa, Yoji ; Todokoro, Kazuo. / Constitutive expression of exogenous c-myb gene causes maturation block in monocyte-macrophage differentiation. In: BBA - Gene Structure and Expression. 1991 ; Vol. 1088, No. 3. pp. 380-384.
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