Constitutive expression of exogenous c-myb gene causes maturation block in monocyte-macrophage differentiation

Hiromi Yanagisawa, Toshiro Nagasawa, Satomi Kuramochi, Tsukasa Abe, Yoji Ikawa, Kazuo Todokoro

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

A nuclear protooncogene c-myb has been hypothesized to play an important role in hematopoiesis, but little is known about the physiological function of the c-myb gene products. To study the role of c-myb gene expression in monocyte-macrophage differentiation and proliferation, we introduced exogenous c-myb gene into murine myelomonocytic leukemia WEHI-3B(D+) cells which can be induced to differentiate into mature monocytes with granulocyte-colony stimulation factor (G-CSF) and actinomycin D. Expression of the transfected gene was found to result in elevated levels of c-myb transcripts, which were not subject to normal down-regulation by differentiation induction. This constitutive expression of c-myb gene allowed the c-myb transfectants to differentiate into promonocytes with G-CSF and actinomycin D, but blocked further maturation from promonocytes to mature monocytes. It is concluded that normal down-regulation of c-myb gene expression during monocyte-macrophage differentiation is required for the maturation of promonocytes to mature monocytes.

Original languageEnglish (US)
Pages (from-to)380-384
Number of pages5
JournalBBA - Gene Structure and Expression
Volume1088
Issue number3
DOIs
StatePublished - Mar 26 1991

Keywords

  • Differentiation
  • Granulocyte colony stimulation factor
  • Maturation
  • Myelomonocyte
  • c-myb

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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