Abstract
A nuclear protooncogene c-myb has been hypothesized to play an important role in hematopoiesis, but little is known about the physiological function of the c-myb gene products. To study the role of c-myb gene expression in monocyte-macrophage differentiation and proliferation, we introduced exogenous c-myb gene into murine myelomonocytic leukemia WEHI-3B(D+) cells which can be induced to differentiate into mature monocytes with granulocyte-colony stimulation factor (G-CSF) and actinomycin D. Expression of the transfected gene was found to result in elevated levels of c-myb transcripts, which were not subject to normal down-regulation by differentiation induction. This constitutive expression of c-myb gene allowed the c-myb transfectants to differentiate into promonocytes with G-CSF and actinomycin D, but blocked further maturation from promonocytes to mature monocytes. It is concluded that normal down-regulation of c-myb gene expression during monocyte-macrophage differentiation is required for the maturation of promonocytes to mature monocytes.
Original language | English (US) |
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Pages (from-to) | 380-384 |
Number of pages | 5 |
Journal | BBA - Gene Structure and Expression |
Volume | 1088 |
Issue number | 3 |
DOIs | |
State | Published - Mar 26 1991 |
Keywords
- Differentiation
- Granulocyte colony stimulation factor
- Maturation
- Myelomonocyte
- c-myb
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics