Construction of 110 cosmid markers and a 4.5-MB YAC contig on human chromosome 8p12-q11

Akihiro Kurimasa; Noriyuki Suzuki; Satoshi Kumano; Hua Li; Dan Wells; Michael J. Wagner; Fanqing Chen; David J. Chen; Mitsuo Oshimura

doi:10.1006/geno.1995.1125

Construction of 110 cosmid markers and a 4.5-MB YAC contig on human chromosome 8p12-q11

Akihiro Kurimasa, Noriyuki Suzuki, Satoshi Kumano, Hua Li, Dan Wells, Michael J. Wagner, Fanqing Chen, David J. Chen, Mitsuo Oshimura

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Microcell hybrids containing various regions of human chromosome 8 were formed by microcell-mediated transfer of neo-tagged chromosome 8 into the cells derived from severe combined immunodeficiency (SCID) mouse. Thus, 110 cosmid markers were isolated from SV40-transformed SCID fibroblast cell line (SCVA) containing a p12-q11.1 region of human chromosome 8 and were assigned to eight regions in 8p12-q11.1, using a microcell-hybrid panel. For positional cloning of a human gene that restores the DNA-repair defect in a mouse with SCID on 8p11.1-q11.1 (SCID region), we constructed a yeast artificial chromosome (YAC) contig of about 4.5 Mb. Overlapping YACs were further aligned by restriction mapping, using rare-cutting restriction endonucleases. The cosmids and YAC contig should facilitate isolation of the SCID gene and other genes, such as the Werner syndrome-responsible gene in or near this region.

Original language	English (US)
Pages (from-to)	147-153
Number of pages	7
Journal	Genomics
Volume	28
Issue number	2
DOIs	https://doi.org/10.1006/geno.1995.1125
State	Published - Jul 20 1995

ASJC Scopus subject areas

Genetics

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title = "Construction of 110 cosmid markers and a 4.5-MB YAC contig on human chromosome 8p12-q11",

abstract = "Microcell hybrids containing various regions of human chromosome 8 were formed by microcell-mediated transfer of neo-tagged chromosome 8 into the cells derived from severe combined immunodeficiency (SCID) mouse. Thus, 110 cosmid markers were isolated from SV40-transformed SCID fibroblast cell line (SCVA) containing a p12-q11.1 region of human chromosome 8 and were assigned to eight regions in 8p12-q11.1, using a microcell-hybrid panel. For positional cloning of a human gene that restores the DNA-repair defect in a mouse with SCID on 8p11.1-q11.1 (SCID region), we constructed a yeast artificial chromosome (YAC) contig of about 4.5 Mb. Overlapping YACs were further aligned by restriction mapping, using rare-cutting restriction endonucleases. The cosmids and YAC contig should facilitate isolation of the SCID gene and other genes, such as the Werner syndrome-responsible gene in or near this region.",

author = "Akihiro Kurimasa and Noriyuki Suzuki and Satoshi Kumano and Hua Li and Dan Wells and Wagner, {Michael J.} and Fanqing Chen and Chen, {David J.} and Mitsuo Oshimura",

year = "1995",

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doi = "10.1006/geno.1995.1125",

language = "English (US)",

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AU - Kurimasa, Akihiro

AU - Suzuki, Noriyuki

AU - Kumano, Satoshi

AU - Li, Hua

AU - Wells, Dan

AU - Wagner, Michael J.

AU - Chen, Fanqing

AU - Chen, David J.

AU - Oshimura, Mitsuo

PY - 1995/7/20

Y1 - 1995/7/20

N2 - Microcell hybrids containing various regions of human chromosome 8 were formed by microcell-mediated transfer of neo-tagged chromosome 8 into the cells derived from severe combined immunodeficiency (SCID) mouse. Thus, 110 cosmid markers were isolated from SV40-transformed SCID fibroblast cell line (SCVA) containing a p12-q11.1 region of human chromosome 8 and were assigned to eight regions in 8p12-q11.1, using a microcell-hybrid panel. For positional cloning of a human gene that restores the DNA-repair defect in a mouse with SCID on 8p11.1-q11.1 (SCID region), we constructed a yeast artificial chromosome (YAC) contig of about 4.5 Mb. Overlapping YACs were further aligned by restriction mapping, using rare-cutting restriction endonucleases. The cosmids and YAC contig should facilitate isolation of the SCID gene and other genes, such as the Werner syndrome-responsible gene in or near this region.

AB - Microcell hybrids containing various regions of human chromosome 8 were formed by microcell-mediated transfer of neo-tagged chromosome 8 into the cells derived from severe combined immunodeficiency (SCID) mouse. Thus, 110 cosmid markers were isolated from SV40-transformed SCID fibroblast cell line (SCVA) containing a p12-q11.1 region of human chromosome 8 and were assigned to eight regions in 8p12-q11.1, using a microcell-hybrid panel. For positional cloning of a human gene that restores the DNA-repair defect in a mouse with SCID on 8p11.1-q11.1 (SCID region), we constructed a yeast artificial chromosome (YAC) contig of about 4.5 Mb. Overlapping YACs were further aligned by restriction mapping, using rare-cutting restriction endonucleases. The cosmids and YAC contig should facilitate isolation of the SCID gene and other genes, such as the Werner syndrome-responsible gene in or near this region.

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JO - Genomics

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Construction of 110 cosmid markers and a 4.5-MB YAC contig on human chromosome 8p12-q11

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