Construction of 110 cosmid markers and a 4.5-MB YAC contig on human chromosome 8p12-q11

Akihiro Kurimasa, Noriyuki Suzuki, Satoshi Kumano, Hua Li, Dan Wells, Michael J. Wagner, Fanqing Chen, David J. Chen, Mitsuo Oshimura

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Microcell hybrids containing various regions of human chromosome 8 were formed by microcell-mediated transfer of neo-tagged chromosome 8 into the cells derived from severe combined immunodeficiency (SCID) mouse. Thus, 110 cosmid markers were isolated from SV40-transformed SCID fibroblast cell line (SCVA) containing a p12-q11.1 region of human chromosome 8 and were assigned to eight regions in 8p12-q11.1, using a microcell-hybrid panel. For positional cloning of a human gene that restores the DNA-repair defect in a mouse with SCID on 8p11.1-q11.1 (SCID region), we constructed a yeast artificial chromosome (YAC) contig of about 4.5 Mb. Overlapping YACs were further aligned by restriction mapping, using rare-cutting restriction endonucleases. The cosmids and YAC contig should facilitate isolation of the SCID gene and other genes, such as the Werner syndrome-responsible gene in or near this region.

Original languageEnglish (US)
Pages (from-to)147-153
Number of pages7
JournalGenomics
Volume28
Issue number2
DOIs
StatePublished - Jul 20 1995

ASJC Scopus subject areas

  • Genetics

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    Kurimasa, A., Suzuki, N., Kumano, S., Li, H., Wells, D., Wagner, M. J., Chen, F., Chen, D. J., & Oshimura, M. (1995). Construction of 110 cosmid markers and a 4.5-MB YAC contig on human chromosome 8p12-q11. Genomics, 28(2), 147-153. https://doi.org/10.1006/geno.1995.1125