Contact chemoreceptors mediate male-male repulsion and male-female attraction during drosophila courtship

Robert Thistle, Peter Cameron, Azeen Ghorayshi, Lisa Dennison, Kristin Scott

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The elaborate courtship ritual of Drosophila males is dictated by neural circuitry established by the transcription factor Fruitless and triggered by sex-specific sensory cues. Deciphering the role of different stimuli in driving courtship behavior has been limited by the inability to selectively target appropriate sensory classes. Here, we identify two ion channel genes belonging to the degenerin/epithelial sodium channel/pickpocket (ppk) family, ppk23 and ppk29, which are expressed in fruitless-positive neurons on the legs and are essential for courtship. Gene loss-of-function, cell-inactivation, and cell-activation experiments demonstrate that these genes and neurons are necessary and sufficient to inhibit courtship toward males and promote courtship toward females. Moreover, these cells respond to cuticular hydrocarbons, with different cells selectively responding to male or female pheromones. These studies identify a large population of pheromone-sensing neurons and demonstrate the essential role of contact chemosensation in the early courtship steps of mate selection and courtship initiation.

Original languageEnglish (US)
Pages (from-to)1140-1151
Number of pages12
JournalCell
Volume149
Issue number5
DOIs
StatePublished - May 25 2012
Externally publishedYes

Fingerprint

Courtship
Drosophila
Neurons
Genes
Pheromones
Degenerin Sodium Channels
Epithelial Sodium Channels
Hydrocarbons
Ion Channels
Transcription Factors
Chemical activation
Ceremonial Behavior
Marriage
Cues
Experiments
Leg
Population

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Contact chemoreceptors mediate male-male repulsion and male-female attraction during drosophila courtship. / Thistle, Robert; Cameron, Peter; Ghorayshi, Azeen; Dennison, Lisa; Scott, Kristin.

In: Cell, Vol. 149, No. 5, 25.05.2012, p. 1140-1151.

Research output: Contribution to journalArticle

Thistle, Robert ; Cameron, Peter ; Ghorayshi, Azeen ; Dennison, Lisa ; Scott, Kristin. / Contact chemoreceptors mediate male-male repulsion and male-female attraction during drosophila courtship. In: Cell. 2012 ; Vol. 149, No. 5. pp. 1140-1151.
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