Containment of herbicide resistance through genetic engineering of the chloroplast genome

Henry Daniell, Rina Datta, Sam Varma, Steven Gray, Seung Bum Lee

Research output: Contribution to journalArticle

291 Citations (Scopus)

Abstract

Glyphosate is a potent herbicide. It works by competitive inhibition of the enzyme 5-enol-pyruvyl shiki-mate-3-phosphate synthase (EPSPS), which catalyzes an essential step in the aromatic amino acid biosynthetic pathway. We report the genetic engineering of herbicide resistance by stable integration of the petunia EPSPS gene into the tobacco chloroplast genome using the tobacco or universal vector. Southern blot analysis confirms stable integration of the EPSPS pone into all of the chloroplast genomes (5000- 10,000 copies per cell) of transgenic plants. Seeds obtained after the first self-cross of transgenic plants germinated and grew normally in the presence of the selectable marker, whereas the control seedlings were bleached. While control plants were extremely sensitive to glyphosate, transgenic plants survived sprays of high concentrations of glyphosate. Chloroplast transformation provides containment of foreign genes because plastid transgenes are not transmitted by pollen. The escape of foreign genes via pollen is a serious environmental concern in nuclear transgenic plants because of the high rates of gene flow from crops to wild weedy relatives.

Original languageEnglish (US)
Pages (from-to)345-348
Number of pages4
JournalNature Biotechnology
Volume16
Issue number4
DOIs
StatePublished - Apr 1 1998

Fingerprint

glyphosate
Herbicide Resistance
Chloroplast Genome
Genetic engineering
Genetic Engineering
Herbicides
Genetically Modified Plants
Genes
Phosphates
Pollen
Tobacco
Petunia
Aromatic Amino Acids
Plastids
Gene Flow
Biosynthetic Pathways
Chloroplasts
Southern Blotting
Transgenes
Seedlings

Keywords

  • Agricultural biotechnology
  • Glyphosate
  • Tobacco
  • Transformation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Molecular Medicine

Cite this

Containment of herbicide resistance through genetic engineering of the chloroplast genome. / Daniell, Henry; Datta, Rina; Varma, Sam; Gray, Steven; Lee, Seung Bum.

In: Nature Biotechnology, Vol. 16, No. 4, 01.04.1998, p. 345-348.

Research output: Contribution to journalArticle

Daniell, Henry ; Datta, Rina ; Varma, Sam ; Gray, Steven ; Lee, Seung Bum. / Containment of herbicide resistance through genetic engineering of the chloroplast genome. In: Nature Biotechnology. 1998 ; Vol. 16, No. 4. pp. 345-348.
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