Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor

A. F. Gazdar, W. L. Chick, H. K. Oie, H. L. Sims, D. L. King, G. C. Weir, V. Lauris

Research output: Contribution to journalArticle

432 Citations (Scopus)

Abstract

Continuous cell lines that secrete both insulin and somatostatin were established by two cooperating groups of investigators from a serially transplantable, radiation-induced, rat islet cell tumor. The cell lines, named RIN-r and RIN-m, were initiated from tumors maintained in inbred rats or in athymic nude mice, respectively. The cultured cells are epithelioid, free of fibroblast contamination, and can be cloned. They have a hypodiploid chromosome number, are tumorigenic, and possess amine-handling properties, including high levels of L-dopa decarboxylase and formaldehyde-induced fluorescence. Preliminary analysis of clones revealed a spectrum of insulin secretion from undetectable to relatively high. These clonal cell lines provide important systems to study the biology of insulin and somatostatin.

Original languageEnglish (US)
Pages (from-to)3519-3523
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number6 I
DOIs
StatePublished - 1980

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Islet Cell Adenoma
Somatostatin-Secreting Cells
Insulin-Secreting Cells
Insulin
Somatostatin
Nude Mice
Cell Line
Dopa Decarboxylase
Levodopa
Formaldehyde
Amines
Cultured Cells
Clone Cells
Fibroblasts
Chromosomes
Fluorescence
Research Personnel
Radiation
Neoplasms

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor. / Gazdar, A. F.; Chick, W. L.; Oie, H. K.; Sims, H. L.; King, D. L.; Weir, G. C.; Lauris, V.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 6 I, 1980, p. 3519-3523.

Research output: Contribution to journalArticle

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AU - Sims, H. L.

AU - King, D. L.

AU - Weir, G. C.

AU - Lauris, V.

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