Continuous contractile activity induces fiber type specific expression of HSP70 in skeletal muscle

P. Darrell Neufer, George A. Ordway, Gregory A. Hand, John M. Shelton, James A. Richardson, Ivor J. Benjamin, R. Sanders Williams

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Continuous contractile activity of skeletal muscle elicits an early and dramatic increase in ribosomal RNA, suggesting that translational efficiency and/or capacity is enhanced during the adaptive response to increased metabolic demand. In view of the important role heat shock or stress proteins (HSPs) play as molecular chaperones during protein synthesis, we examined whether expression of the inducible 70-kDa HSP (HSP70) and/or mitochondrial 60-kDa HSP (HSP60) is altered in rabbit tibialis anterior muscle during continuous low-frequency motor nerve stimulation. Induction of the HSP70 gene was evident within 24 h after the onset of stimulation as reflected by increases in HSP70 transcription (>20-fold) and mRNA (>50-fold). HSP70 protein levels were significantly elevated (10- to 12-fold) after 14 and 21 days of stimulation. Mitochondrial HSP60 mRNA and protein also increased during stimulation (>18- and >5-fold after 21 days, respectively). In situ hybridization and immunohistochemistry coupled with myosin ATPase staining revealed that expression of HSP70 was restricted to oxidative type I and IIa fibers during the first 3 days of stimulation but shifted to primarily type II fibers after 21 days of stimulation. These findings demonstrate that induction of HSP70 during the adaptive response to chronic motor nerve stimulation proceeds from type I/IIa to type IId(x)/b fibers, suggesting that the expression of HSPs may be required to support the folding and compartmentalization of nascent proteins during the transformation process.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume271
Issue number6 40-6
StatePublished - Dec 1996

Fingerprint

Heat-Shock Proteins
Muscle
Skeletal Muscle
Fibers
Proteins
Messenger RNA
Molecular Chaperones
Ribosomal RNA
Mitochondrial Proteins
Transcription
Myosins
In Situ Hybridization
Genes
Immunohistochemistry
Staining and Labeling
Rabbits
Muscles

Keywords

  • exercise
  • gene expression
  • stress proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Neufer, P. D., Ordway, G. A., Hand, G. A., Shelton, J. M., Richardson, J. A., Benjamin, I. J., & Williams, R. S. (1996). Continuous contractile activity induces fiber type specific expression of HSP70 in skeletal muscle. American Journal of Physiology - Cell Physiology, 271(6 40-6).

Continuous contractile activity induces fiber type specific expression of HSP70 in skeletal muscle. / Neufer, P. Darrell; Ordway, George A.; Hand, Gregory A.; Shelton, John M.; Richardson, James A.; Benjamin, Ivor J.; Williams, R. Sanders.

In: American Journal of Physiology - Cell Physiology, Vol. 271, No. 6 40-6, 12.1996.

Research output: Contribution to journalArticle

Neufer, PD, Ordway, GA, Hand, GA, Shelton, JM, Richardson, JA, Benjamin, IJ & Williams, RS 1996, 'Continuous contractile activity induces fiber type specific expression of HSP70 in skeletal muscle', American Journal of Physiology - Cell Physiology, vol. 271, no. 6 40-6.
Neufer, P. Darrell ; Ordway, George A. ; Hand, Gregory A. ; Shelton, John M. ; Richardson, James A. ; Benjamin, Ivor J. ; Williams, R. Sanders. / Continuous contractile activity induces fiber type specific expression of HSP70 in skeletal muscle. In: American Journal of Physiology - Cell Physiology. 1996 ; Vol. 271, No. 6 40-6.
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