Control of human papillomavirus type 11 origin of replication by the E2 family of transcription regulatory proteins

Cheng Ming Chiang, Gang Dong, Thomas R. Broker, Louise T. Chow

Research output: Contribution to journalArticle

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Abstract

Replication of human papillomavirus type 11 (HPV-11) DNA requires the full-length viral E1 and E2 proteins (C.-M. Chiang, M. Ustav, A. Stenlund, T. F. Ho, T. R. Broker, and L. T. Chow, Proc. Natl. Acad. Sci. USA 89:5799-5803, 1992). Using transient transfection of subgenomic HPV DNA into hamster CHO and human 293 cells, we have localized an origin of replication (ori) to an 80-bp segment in the upstream regulatory region spanning nucleotide 1. It overlaps the E6 promoter region and contains a short A+T-rich segment and a sequence which is homologous to the binding site of the bovine papillomavirus type 1 (BPV-1) E1 protein in the BPV-1 ori. However, unlike the BPV-1 ori, for which half an E2-responsive sequence (E2-RS) or binding site suffices, an intact binding site is essential for the HPV-11 ori. Replication was more efficient when additional E2-RSs were present. The intact HPV-11 genome also replicated in both cell lines when supplied with E1 and E2 proteins. Expression vectors of transcription repressor proteins that lack the N-terminal domain essential for E2 transcriptional trans activation did not support replication in collaboration with the E1 expression vector. Rather, cotransfection with the repressor expression vectors inhibited ori replication by the E1 and E2 proteins. These results demonstrate the importance of the N-terminal domain of the E2 protein in DNA replication and indicate that the family of E2 proteins positively and negatively regulates both viral DNA replication and E6 promoter transcription.

Original languageEnglish (US)
Pages (from-to)5224-5231
Number of pages8
JournalJournal of Virology
Volume66
Issue number9
StatePublished - Sep 1992

Fingerprint

Human papillomavirus 11
replication origin
Replication Origin
Papillomaviridae
regulatory proteins
Bovine papillomavirus 1
transcription (genetics)
Binding Sites
binding sites
Proteins
DNA Replication
proteins
DNA replication
Repressor Proteins
promoter regions
Nucleic Acid Regulatory Sequences
DNA
Viral DNA
Protein C
Genetic Promoter Regions

ASJC Scopus subject areas

  • Immunology

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Control of human papillomavirus type 11 origin of replication by the E2 family of transcription regulatory proteins. / Chiang, Cheng Ming; Dong, Gang; Broker, Thomas R.; Chow, Louise T.

In: Journal of Virology, Vol. 66, No. 9, 09.1992, p. 5224-5231.

Research output: Contribution to journalArticle

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