Control of muscle formation by the fusogenic micropeptide myomixer

Pengpeng Bi, Andres Ramirez-Martinez, Hui Li, Jessica Cannavino, John R. McAnally, John M. Shelton, Efrain Sánchez-Ortiz, Rhonda Bassel-Duby, Eric N. Olson

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Skeletal muscle formation occurs through fusion of myoblasts to form multinucleated myofibers. From a genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) loss-of-function screen for genes required for myoblast fusion and myogenesis, we discovered an 84-amino acid muscle-specific peptide that we call Myomixer. Myomixer expression coincides with myoblast differentiation and is essential for fusion and skeletal muscle formation during embryogenesis. Myomixer localizes to the plasma membrane, where it promotes myoblast fusion and associates with Myomaker, a fusogenic membrane protein. Myomixer together with Myomaker can also induce fibroblast-fibroblast fusion and fibroblast-myoblast fusion.We conclude that the Myomixer-Myomaker pair controls the critical step in myofiber formation during muscle development.

Original languageEnglish (US)
Pages (from-to)323-327
Number of pages5
JournalScience
Volume356
Issue number6335
DOIs
StatePublished - Apr 21 2017

Fingerprint

Myoblasts
Muscles
Muscle Development
Fibroblasts
Skeletal Muscle
Clustered Regularly Interspaced Short Palindromic Repeats
Embryonic Development
Membrane Proteins
Cell Membrane
Genome
Amino Acids
Peptides
Genes

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Bi, P., Ramirez-Martinez, A., Li, H., Cannavino, J., McAnally, J. R., Shelton, J. M., ... Olson, E. N. (2017). Control of muscle formation by the fusogenic micropeptide myomixer. Science, 356(6335), 323-327. https://doi.org/10.1126/science.aam9361

Control of muscle formation by the fusogenic micropeptide myomixer. / Bi, Pengpeng; Ramirez-Martinez, Andres; Li, Hui; Cannavino, Jessica; McAnally, John R.; Shelton, John M.; Sánchez-Ortiz, Efrain; Bassel-Duby, Rhonda; Olson, Eric N.

In: Science, Vol. 356, No. 6335, 21.04.2017, p. 323-327.

Research output: Contribution to journalArticle

Bi, P, Ramirez-Martinez, A, Li, H, Cannavino, J, McAnally, JR, Shelton, JM, Sánchez-Ortiz, E, Bassel-Duby, R & Olson, EN 2017, 'Control of muscle formation by the fusogenic micropeptide myomixer', Science, vol. 356, no. 6335, pp. 323-327. https://doi.org/10.1126/science.aam9361
Bi P, Ramirez-Martinez A, Li H, Cannavino J, McAnally JR, Shelton JM et al. Control of muscle formation by the fusogenic micropeptide myomixer. Science. 2017 Apr 21;356(6335):323-327. https://doi.org/10.1126/science.aam9361
Bi, Pengpeng ; Ramirez-Martinez, Andres ; Li, Hui ; Cannavino, Jessica ; McAnally, John R. ; Shelton, John M. ; Sánchez-Ortiz, Efrain ; Bassel-Duby, Rhonda ; Olson, Eric N. / Control of muscle formation by the fusogenic micropeptide myomixer. In: Science. 2017 ; Vol. 356, No. 6335. pp. 323-327.
@article{79b599312dee49ee8ce2a2c578bf653b,
title = "Control of muscle formation by the fusogenic micropeptide myomixer",
abstract = "Skeletal muscle formation occurs through fusion of myoblasts to form multinucleated myofibers. From a genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) loss-of-function screen for genes required for myoblast fusion and myogenesis, we discovered an 84-amino acid muscle-specific peptide that we call Myomixer. Myomixer expression coincides with myoblast differentiation and is essential for fusion and skeletal muscle formation during embryogenesis. Myomixer localizes to the plasma membrane, where it promotes myoblast fusion and associates with Myomaker, a fusogenic membrane protein. Myomixer together with Myomaker can also induce fibroblast-fibroblast fusion and fibroblast-myoblast fusion.We conclude that the Myomixer-Myomaker pair controls the critical step in myofiber formation during muscle development.",
author = "Pengpeng Bi and Andres Ramirez-Martinez and Hui Li and Jessica Cannavino and McAnally, {John R.} and Shelton, {John M.} and Efrain S{\'a}nchez-Ortiz and Rhonda Bassel-Duby and Olson, {Eric N.}",
year = "2017",
month = "4",
day = "21",
doi = "10.1126/science.aam9361",
language = "English (US)",
volume = "356",
pages = "323--327",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6335",

}

TY - JOUR

T1 - Control of muscle formation by the fusogenic micropeptide myomixer

AU - Bi, Pengpeng

AU - Ramirez-Martinez, Andres

AU - Li, Hui

AU - Cannavino, Jessica

AU - McAnally, John R.

AU - Shelton, John M.

AU - Sánchez-Ortiz, Efrain

AU - Bassel-Duby, Rhonda

AU - Olson, Eric N.

PY - 2017/4/21

Y1 - 2017/4/21

N2 - Skeletal muscle formation occurs through fusion of myoblasts to form multinucleated myofibers. From a genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) loss-of-function screen for genes required for myoblast fusion and myogenesis, we discovered an 84-amino acid muscle-specific peptide that we call Myomixer. Myomixer expression coincides with myoblast differentiation and is essential for fusion and skeletal muscle formation during embryogenesis. Myomixer localizes to the plasma membrane, where it promotes myoblast fusion and associates with Myomaker, a fusogenic membrane protein. Myomixer together with Myomaker can also induce fibroblast-fibroblast fusion and fibroblast-myoblast fusion.We conclude that the Myomixer-Myomaker pair controls the critical step in myofiber formation during muscle development.

AB - Skeletal muscle formation occurs through fusion of myoblasts to form multinucleated myofibers. From a genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) loss-of-function screen for genes required for myoblast fusion and myogenesis, we discovered an 84-amino acid muscle-specific peptide that we call Myomixer. Myomixer expression coincides with myoblast differentiation and is essential for fusion and skeletal muscle formation during embryogenesis. Myomixer localizes to the plasma membrane, where it promotes myoblast fusion and associates with Myomaker, a fusogenic membrane protein. Myomixer together with Myomaker can also induce fibroblast-fibroblast fusion and fibroblast-myoblast fusion.We conclude that the Myomixer-Myomaker pair controls the critical step in myofiber formation during muscle development.

UR - http://www.scopus.com/inward/record.url?scp=85017248643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85017248643&partnerID=8YFLogxK

U2 - 10.1126/science.aam9361

DO - 10.1126/science.aam9361

M3 - Article

C2 - 28386024

AN - SCOPUS:85017248643

VL - 356

SP - 323

EP - 327

JO - Science

JF - Science

SN - 0036-8075

IS - 6335

ER -