TY - JOUR
T1 - Control of muscle formation by the fusogenic micropeptide myomixer
AU - Bi, Pengpeng
AU - Ramirez-Martinez, Andres
AU - Li, Hui
AU - Cannavino, Jessica
AU - McAnally, John R.
AU - Shelton, John M.
AU - Sánchez-Ortiz, Efrain
AU - Bassel-Duby, Rhonda
AU - Olson, Eric N.
PY - 2017/4/21
Y1 - 2017/4/21
N2 - Skeletal muscle formation occurs through fusion of myoblasts to form multinucleated myofibers. From a genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) loss-of-function screen for genes required for myoblast fusion and myogenesis, we discovered an 84-amino acid muscle-specific peptide that we call Myomixer. Myomixer expression coincides with myoblast differentiation and is essential for fusion and skeletal muscle formation during embryogenesis. Myomixer localizes to the plasma membrane, where it promotes myoblast fusion and associates with Myomaker, a fusogenic membrane protein. Myomixer together with Myomaker can also induce fibroblast-fibroblast fusion and fibroblast-myoblast fusion.We conclude that the Myomixer-Myomaker pair controls the critical step in myofiber formation during muscle development.
AB - Skeletal muscle formation occurs through fusion of myoblasts to form multinucleated myofibers. From a genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) loss-of-function screen for genes required for myoblast fusion and myogenesis, we discovered an 84-amino acid muscle-specific peptide that we call Myomixer. Myomixer expression coincides with myoblast differentiation and is essential for fusion and skeletal muscle formation during embryogenesis. Myomixer localizes to the plasma membrane, where it promotes myoblast fusion and associates with Myomaker, a fusogenic membrane protein. Myomixer together with Myomaker can also induce fibroblast-fibroblast fusion and fibroblast-myoblast fusion.We conclude that the Myomixer-Myomaker pair controls the critical step in myofiber formation during muscle development.
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U2 - 10.1126/science.aam9361
DO - 10.1126/science.aam9361
M3 - Article
C2 - 28386024
AN - SCOPUS:85017248643
SN - 0036-8075
VL - 356
SP - 323
EP - 327
JO - Science
JF - Science
IS - 6335
ER -