Control of stromal keratitis by inhibition of neovascularization

Mei Zheng, Margaret A. Schwarz, Sujin Lee, Uday Kumaraguru, Barry T. Rouse

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Stromal keratitis resulting from ocular infection with herpes simplex virus is a common cause of blindness. This report investigates the role of neovascularization in the pathogenesis of stromal keratitis by measuring the outcome of treatment with the potent anti-angiogenesis cytokine endothelial monocyte-activating polypeptide II (EMAP II). We show that systemic and topical administration of EMAP II from the outset of infection resulted in markedly diminished levels of herpes simplex virus-induced angiogenesis and significantly reduced the severity of stromal keratitis lesions. EMAP II treatment had no demonstrable proinflammatory or toxic effects and failed to express antiviral activity. The mechanism of action of EMAP II was shown to proceed by causing apoptosis in vascular endothelial cells. Our data document for the first time the essential role of angiogenesis in the pathogenesis of stromal keratitis and also indicate that the therapy of herpetic stromal keratitis could benefit by procedures that diminish angiogenesis.

Original languageEnglish (US)
Pages (from-to)1021-1029
Number of pages9
JournalAmerican Journal of Pathology
Volume159
Issue number3
StatePublished - 2001

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Keratitis
Simplexvirus
Herpetic Keratitis
Eye Infections
Topical Administration
Poisons
Blindness
Antiviral Agents
Endothelial Cells
Apoptosis
Cytokines
small inducible cytokine subfamily E, member 1
Therapeutics
Infection

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Zheng, M., Schwarz, M. A., Lee, S., Kumaraguru, U., & Rouse, B. T. (2001). Control of stromal keratitis by inhibition of neovascularization. American Journal of Pathology, 159(3), 1021-1029.

Control of stromal keratitis by inhibition of neovascularization. / Zheng, Mei; Schwarz, Margaret A.; Lee, Sujin; Kumaraguru, Uday; Rouse, Barry T.

In: American Journal of Pathology, Vol. 159, No. 3, 2001, p. 1021-1029.

Research output: Contribution to journalArticle

Zheng, M, Schwarz, MA, Lee, S, Kumaraguru, U & Rouse, BT 2001, 'Control of stromal keratitis by inhibition of neovascularization', American Journal of Pathology, vol. 159, no. 3, pp. 1021-1029.
Zheng M, Schwarz MA, Lee S, Kumaraguru U, Rouse BT. Control of stromal keratitis by inhibition of neovascularization. American Journal of Pathology. 2001;159(3):1021-1029.
Zheng, Mei ; Schwarz, Margaret A. ; Lee, Sujin ; Kumaraguru, Uday ; Rouse, Barry T. / Control of stromal keratitis by inhibition of neovascularization. In: American Journal of Pathology. 2001 ; Vol. 159, No. 3. pp. 1021-1029.
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