Convective movement of Ca2+ across guinea pig gallbladder epithelium

Robert V Rege, E. W. Moore

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recently, much interest has developed in biliary calcium because of its importance in the pathogenesis and composition of gallstones. While much progress has been made in understanding the thermodynamic factors that control biliary calcium concentrations, little is known about the kinetic factors that control the movement of calcium across the gallbladder epithelium. These studies measure guinea pig gallbladder epithelial permeability to Ca2+ during in vivo convective water movement across the membrane. Water movement, ranging from -15.2 (absorption) to 6.3 μl · min- 1 · cm-2 (water entry), was induced by placing hypotonic, isotonic, and hypertonic solutions into the gallbladder lumen. Calcium movement was found to be directly and linearly related to water flow, indicating that Ca2+ moved with the convective water flow, presumably across paracellular channels. The slope of this relationship (0.602), representing the concentration of calcium in the fluid translocated across the gallbladder epithelium, was only about half that of plasma or luminal contents, indicating that calcium movement across the membrane was restricted. The mean sieving coefficient (1 - r) of guinea pig gallbladder, calculated from this slope, was ~0.5, indicating that the epithelium is only moderately permeable to Ca2+. The results suggest that intraluminal chelation of Ca2+ for the possible prevention and/or treatment of calcium-containing gallstones is a potentially feasible therapeutic modality.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume262
Issue number6 25-6
StatePublished - 1992

Fingerprint

Gallbladder
Guinea Pigs
Epithelium
Calcium
Water Movements
Gallstones
Water
Hypotonic Solutions
Hypertonic Solutions
Isotonic Solutions
Membranes
Thermodynamics
Permeability

Keywords

  • bile composition
  • biliary calcium
  • calcium absorption
  • calcium ion electrode
  • gallstones
  • membrane transport
  • permeability
  • reflection coefficient
  • sieving coefficient
  • solvent drag

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology
  • Medicine(all)

Cite this

Convective movement of Ca2+ across guinea pig gallbladder epithelium. / Rege, Robert V; Moore, E. W.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 262, No. 6 25-6, 1992.

Research output: Contribution to journalArticle

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