Coordinate expression of secretory phospholipase A2 and cyclooxygenase- 2 in activated human keratinocytes

Krystyna E. Rys-Sikora, Raymond L. Konger, John W. Schoggins, Rama Malaviya, Alice P. Pentland

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Abstract

PGE2 levels are altered in human epidermis after in vivo wounding; however, mechanisms modulating PGE2 production in activated keratinocytes are unclear. In previous studies, we showed that PGE2 is a growth-promoting autacoid in human primary keratinocyte cultures, and its production is modulated by plating density, suggesting that regulated PGE2 synthesis is an important component of wound healing. Here, we examine the role of phospholipase A2 (PLA2) and cyclooxygenase (COX) enzymes in modulation of PGE2 production. We report that the increased PGE2 production that occurs in keratinocytes grown in nonconfluent conditions is also observed after in vitro wounding, indicating that similar mechanisms are involved. This increase was associated with coordinate upregulation of both COX-2 and secretory PLA2 (sPLA2) proteins. Increased sPLA2 activity was also observed. By RT-PCR, we identified the presence of type IIA and type V sPLA2, along with the M-type sPLA2 receptor. Thus the coordinate expression of sPLA2 and COX-2 may be responsible for the increased prostaglandin synthesis in activated keratinocytes during wound repair.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume278
Issue number4 47-4
StatePublished - 2000

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Secretory Phospholipase A2
Cyclooxygenase 2
Keratinocytes
Dinoprostone
Autacoids
Phospholipases A2
Prostaglandin-Endoperoxide Synthases
Plating
Epidermis
Wound Healing
Prostaglandins
Repair
Up-Regulation
Modulation
Polymerase Chain Reaction
Wounds and Injuries
Enzymes
Growth

Keywords

  • Arachidonic acid
  • Primary human keratinocytes
  • Prostaglandin E
  • Secretory phospholipase A receptor
  • Wound healing

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Coordinate expression of secretory phospholipase A2 and cyclooxygenase- 2 in activated human keratinocytes. / Rys-Sikora, Krystyna E.; Konger, Raymond L.; Schoggins, John W.; Malaviya, Rama; Pentland, Alice P.

In: American Journal of Physiology - Cell Physiology, Vol. 278, No. 4 47-4, 2000.

Research output: Contribution to journalArticle

Rys-Sikora, Krystyna E. ; Konger, Raymond L. ; Schoggins, John W. ; Malaviya, Rama ; Pentland, Alice P. / Coordinate expression of secretory phospholipase A2 and cyclooxygenase- 2 in activated human keratinocytes. In: American Journal of Physiology - Cell Physiology. 2000 ; Vol. 278, No. 4 47-4.
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AU - Malaviya, Rama

AU - Pentland, Alice P.

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N2 - PGE2 levels are altered in human epidermis after in vivo wounding; however, mechanisms modulating PGE2 production in activated keratinocytes are unclear. In previous studies, we showed that PGE2 is a growth-promoting autacoid in human primary keratinocyte cultures, and its production is modulated by plating density, suggesting that regulated PGE2 synthesis is an important component of wound healing. Here, we examine the role of phospholipase A2 (PLA2) and cyclooxygenase (COX) enzymes in modulation of PGE2 production. We report that the increased PGE2 production that occurs in keratinocytes grown in nonconfluent conditions is also observed after in vitro wounding, indicating that similar mechanisms are involved. This increase was associated with coordinate upregulation of both COX-2 and secretory PLA2 (sPLA2) proteins. Increased sPLA2 activity was also observed. By RT-PCR, we identified the presence of type IIA and type V sPLA2, along with the M-type sPLA2 receptor. Thus the coordinate expression of sPLA2 and COX-2 may be responsible for the increased prostaglandin synthesis in activated keratinocytes during wound repair.

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