Coordinate regulation of amylin and insulin expression in response to hypoglycemia and fasting

Tausif Alam, Ling Chen, Atsushi Ogawa, Jonathan D. Leffert, Roger H Unger, Kenneth L. Luskey

Research output: Contribution to journalArticle

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Abstract

Amylin is a 37-amino acid peptide synthesized in the pancreatic β-cell and cosecreted with insulin. In situ hybridization of nondiabetic rat pancreas shows that insulin and amylin RNA are both localized within the islet of Langerhans in a similar distribution. After 12 days of insulin-induced hypoglycemia (mean blood glucose 3.0 ± 0.4 mM [54 ± 8 mg/dl]), both insulin and amylin RNA fell >95%. However, maintenance of euglycemia by simultaneous infusion of glucose with insulin did not suppress insulin or amylin RNA. Fasting suppressed amylin and insulin secretion from the isolated, perfused pancreas 70 and 58%, respectively, and with refeeding, secretion rates recovered to fed levels. Despite these changes in the rates of secretion, the relative ratio of amylin to insulin was not significantly different in fed, fasted, or refed rats. The molar ratio of insulin to amylin was estimated to be 100:2.3-2.6. Both insulin and amylin RNA was suppressed ∼50% in response to fasting. Thus, although the absolute amounts of insulin and amylin change substantially under the conditions tested, the relative amounts of these peptides do not change.

Original languageEnglish (US)
Pages (from-to)508-514
Number of pages7
JournalDiabetes
Volume41
Issue number4
StatePublished - Apr 1992

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Islet Amyloid Polypeptide
Hypoglycemia
Insulin
RNA
Pancreas
Fasting
Peptides
Islets of Langerhans
In Situ Hybridization
Blood Glucose

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Alam, T., Chen, L., Ogawa, A., Leffert, J. D., Unger, R. H., & Luskey, K. L. (1992). Coordinate regulation of amylin and insulin expression in response to hypoglycemia and fasting. Diabetes, 41(4), 508-514.

Coordinate regulation of amylin and insulin expression in response to hypoglycemia and fasting. / Alam, Tausif; Chen, Ling; Ogawa, Atsushi; Leffert, Jonathan D.; Unger, Roger H; Luskey, Kenneth L.

In: Diabetes, Vol. 41, No. 4, 04.1992, p. 508-514.

Research output: Contribution to journalArticle

Alam, T, Chen, L, Ogawa, A, Leffert, JD, Unger, RH & Luskey, KL 1992, 'Coordinate regulation of amylin and insulin expression in response to hypoglycemia and fasting', Diabetes, vol. 41, no. 4, pp. 508-514.
Alam T, Chen L, Ogawa A, Leffert JD, Unger RH, Luskey KL. Coordinate regulation of amylin and insulin expression in response to hypoglycemia and fasting. Diabetes. 1992 Apr;41(4):508-514.
Alam, Tausif ; Chen, Ling ; Ogawa, Atsushi ; Leffert, Jonathan D. ; Unger, Roger H ; Luskey, Kenneth L. / Coordinate regulation of amylin and insulin expression in response to hypoglycemia and fasting. In: Diabetes. 1992 ; Vol. 41, No. 4. pp. 508-514.
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