Coordinate regulation of sugar flux and translation by PAS kinase

Jared Rutter, Brandon L. Probst, Steven L. McKnight

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

PAS kinase is a serine/threonine kinase regulated in cis by a PAS domain. A genetic study of the two PAS kinase genes in budding yeast gave evidence of the involvement of these enzymes in the control of sugar metabolism and translation. Using a biochemical screen for PAS kinase substrates, three translation factors were identified as direct phosphorylation targets. PAS kinase was also found to phosphorylate UDP-glucose pyrophosphorylase and glycogen synthase, the enzymes catalyzing the two final steps in the glycogen biosynthetic pathway. Genetic, biochemical, and physiological data provide evidence that both of these enzymes are inhibited by PAS kinase-dependent phosphorylation, thereby downregulating carbohydrate storage. These studies provide evidence of a cell-autonomous signaling system that both controls and connects the balance of fuel consumption/storage to protein synthesis.

Original languageEnglish (US)
Pages (from-to)17-28
Number of pages12
JournalCell
Volume111
Issue number1
DOIs
StatePublished - Oct 4 2002

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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