Corneal allografts induce cytotoxic T cell but not delayed hypersensitivity responses in mice

J. Peeler, J. Niederkorn, A. Matoba

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Abstract

In order to study the cellular immune responses induced by corneal allografts transplanted to a nonimmunologically privileged site, we have used a previously described murine heterotopic corneal allograft model. BALB/c (H-2(d)) corneal allografts transplanted to a vascular graft bed on the thorax of C57BL/6 (H-2(b)) recipients were rapidly rejected within 14 days, while corneal syngrafts survived indefinitely (>28 days). Analysis of both primary and secondary cytotoxic T lymphocyte (CTL) responses 7, 10, 14, and 21 days after heterotopic transplantation revealed antigen-specific CTL reactivity at all time points tested (P<0.01). By contrast, delayed-type hypersensitivity (DTH) responses to corneal alloantigens (footpad swelling assay) were negative (P>0.05) in 83% of the animals tested. The absence of DTH responsiveness to corneal allografts was not a result of the small graft size or antigenic load. Either two or six circular skin allografts (3-mm diam) of BALB/c origin induced both strong CTL and DTH responses (P<0.001) in C57BL/6 recipients, while two or six corneal allografts (2-mm diam) induced strong CTL responses, but DTH responses again failed to develop (P>0.05) in over 75% of the animals tested. The ability of corneal alloantigens to elicit CTL responses but not DTH reactivity when grafted to a heterotopic site provides a basis for understanding the immune privilege enjoyed by this tissue.

Original languageEnglish (US)
Pages (from-to)1516-1523
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume26
Issue number11
StatePublished - 1985

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Delayed Hypersensitivity
Allografts
Cytotoxic T-Lymphocytes
T-Lymphocytes
Heterotopic Transplantation
Transplants
Histocompatibility Antigens
Isoantigens
Cellular Immunity
Blood Vessels
Thorax
Skin

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Corneal allografts induce cytotoxic T cell but not delayed hypersensitivity responses in mice. / Peeler, J.; Niederkorn, J.; Matoba, A.

In: Investigative Ophthalmology and Visual Science, Vol. 26, No. 11, 1985, p. 1516-1523.

Research output: Contribution to journalArticle

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abstract = "In order to study the cellular immune responses induced by corneal allografts transplanted to a nonimmunologically privileged site, we have used a previously described murine heterotopic corneal allograft model. BALB/c (H-2(d)) corneal allografts transplanted to a vascular graft bed on the thorax of C57BL/6 (H-2(b)) recipients were rapidly rejected within 14 days, while corneal syngrafts survived indefinitely (>28 days). Analysis of both primary and secondary cytotoxic T lymphocyte (CTL) responses 7, 10, 14, and 21 days after heterotopic transplantation revealed antigen-specific CTL reactivity at all time points tested (P<0.01). By contrast, delayed-type hypersensitivity (DTH) responses to corneal alloantigens (footpad swelling assay) were negative (P>0.05) in 83{\%} of the animals tested. The absence of DTH responsiveness to corneal allografts was not a result of the small graft size or antigenic load. Either two or six circular skin allografts (3-mm diam) of BALB/c origin induced both strong CTL and DTH responses (P<0.001) in C57BL/6 recipients, while two or six corneal allografts (2-mm diam) induced strong CTL responses, but DTH responses again failed to develop (P>0.05) in over 75{\%} of the animals tested. The ability of corneal alloantigens to elicit CTL responses but not DTH reactivity when grafted to a heterotopic site provides a basis for understanding the immune privilege enjoyed by this tissue.",
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