Corneal epithelial homeostasis following daily and overnight contact lens wear

Patrick M. Ladage, Kazuaki Yamamoto, Ling Li, David H. Ren, Walter M Petroll, James V. Jester, Harrison D Cavanagh

Research output: Contribution to journalArticle

34 Scopus citations


This report reviews and integrates our recent clinical and laboratory findings of the effects of daily (DW)/overnight (EW) contact lens wear on corneal epithelial homeostasis. In a prospective, double-masked human clinical study, three lens types (high and hyper Dk/t soft; hyper Dk/t RGP) were used to assess the effects of daily and overnight lens wear on corneal epithelial thickness, cell surface size, Pseudomonas aeruginosa (PA) binding to exfoliated epithelial cells and exfoliation rates. Concomitantly, we studied in a rabbit model, the effects of overnight lens wear on corneal epithelial surface cell viability (live/dead assay, TUNEL-labeling, Annexin-V staining, Bcl-2 expression), basal cell proliferation and migration patterns. The comprehensive results obtained yield important new insights on both the normal homeostasis of the corneal epithelium and the biological changes induced by contact lens wear.

Original languageEnglish (US)
Pages (from-to)11-21
Number of pages11
JournalContact Lens and Anterior Eye
Issue number1
StatePublished - May 1 2002



  • Annexin-V
  • Apoptosis
  • Bacterial binding
  • Bcl-2
  • Contact lenses
  • Corneal epithelium
  • Exfoliation
  • Proliferation

ASJC Scopus subject areas

  • Ophthalmology
  • Optometry

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