Coupling of mRNA Structure Rearrangement to Ribosome Movement during Bypassing of Non-coding Regions

Jin Chen, Arthur Coakley, Michelle O'Connor, Alexey Petrov, Seán E. O'Leary, John F. Atkins, Joseph D. Puglisi

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Summary Nearly half of the ribosomes translating a particular bacteriophage T4 mRNA bypass a region of 50 nt, resuming translation 3′ of this gap. How this large-scale, specific hop occurs and what determines whether a ribosome bypasses remain unclear. We apply single-molecule fluorescence with zero-mode waveguides to track individual Escherichia coli ribosomes during translation of T4's gene 60 mRNA. Ribosomes that bypass are characterized by a 10- to 20-fold longer pause in a non-canonical rotated state at the take-off codon. During the pause, mRNA secondary structure rearrangements are coupled to ribosome forward movement, facilitated by nascent peptide interactions that disengage the ribosome anticodon-codon interactions for slippage. Close to the landing site, the ribosome then scans mRNA in search of optimal base-pairing interactions. Our results provide a mechanistic and conformational framework for bypassing, highlighting a non-canonical ribosomal state to allow for mRNA structure refolding to drive large-scale ribosome movements.

Original languageEnglish (US)
Pages (from-to)1267-1280
Number of pages14
JournalCell
Volume163
Issue number5
DOIs
StatePublished - Nov 19 2015
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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