Coupling the dynamics of two actin networks - New views on the mechanics of cell protrusion

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

We study how mechanical forces integrate spatially and temporally with regulatory signals at the leading edge of migrating cells. To probe the dynamics of this system, we developed quantitative fluorescent speckle microscopy, which maps out actin cytoskeleton transport, assembly and disassembly with high spatial resolution. Statistical processing of single speckle properties revealed two kinetically, kinematically and molecularly distinct, yet spatially overlapping, actin arrays at the leading edge of migrating epithelial cells. The first network, referred to as the lamellipodium, polymerizes and depolymerizes 1-2 μm from the edge in an Arp2/3 (actin-related protein 2/3)- and cofilin-dependent fashion. The second network, referred to as the lamella, exhibits Arp2/3-independent polymerization. To elucidate the dynamic relationship between the two networks, we have begun to examine how assembly and flow are temporally modulated with respect to a protrusion event. In control cells we found bursts of protrusion preceding bursts of F-actin assembly. The time lag disappears in cells where Arp2/3-function is impaired. This and other results allowed us to propose a model in which tropomyosin protects lamella filaments from branching and severing, and to conjecture that Arp2/3-mediated lamellipodium assembly is a natural consequence of lamella expansion, but not the initiator of cell protrusion.

Original languageEnglish (US)
Pages (from-to)1250-1253
Number of pages4
JournalBiochemical Society Transactions
Volume33
Issue number6
DOIs
StatePublished - Dec 1 2005

Keywords

  • Actin
  • Cell protrusion
  • Epithelial cell
  • Fluorescent speckle microscopy
  • Lamella
  • Lamellipodium

ASJC Scopus subject areas

  • Biochemistry

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