Covalently bound fatty acids in membrane proteins of some sponges

Eser Ayanoglu, Michele Rizzolio, Stacey Beaulieu, Julie Roberts, Orhan Oz, Carl Djerassi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

1. 1. Fatty acids covalently bound to membrane proteins were studied in the marine sponges Spheciospongia confoederata, Microciona prolifera and Tethya aurantia. 2. 2. Cell membrane isolates obtained by successive centrifugation and ultracentrifugation steps were subjected to exhaustive lipid extraction by organic solvents. 3. 3. After delipidation, fatty acids covalently bound to membrane proteins were obtained by hydrolysis. 4. 4. While total lipids from sponge extracts as well as from the organic extracts of the membrane delipidization procedure contained very long chain (C24-30) fatty acids as their major constituents, fatty acids covalently bound to proteins were predominantly of the conventional (C16-18) type.

Original languageEnglish (US)
Pages (from-to)597-603
Number of pages7
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume96
Issue number3
DOIs
StatePublished - 1990

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Porifera
Membrane Proteins
Fatty Acids
Lipids
Centrifugation
Ultracentrifugation
Cell membranes
Organic solvents
Hydrolysis
Cell Membrane
Membranes
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Physiology

Cite this

Covalently bound fatty acids in membrane proteins of some sponges. / Ayanoglu, Eser; Rizzolio, Michele; Beaulieu, Stacey; Roberts, Julie; Oz, Orhan; Djerassi, Carl.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 96, No. 3, 1990, p. 597-603.

Research output: Contribution to journalArticle

Ayanoglu, Eser ; Rizzolio, Michele ; Beaulieu, Stacey ; Roberts, Julie ; Oz, Orhan ; Djerassi, Carl. / Covalently bound fatty acids in membrane proteins of some sponges. In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 1990 ; Vol. 96, No. 3. pp. 597-603.
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