Creating higher titer lentivirus with caffeine

Brian L. Ellis, Patrick Ryan Potts, Matthew H. Porteus

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The use of lentiviral vectors extends from the laboratory, where they are used for basic studies in virology and as gene transfer vectors gene delivery, to the clinic, where clinical trials using these vectors for gene therapy are currently underway. Lentiviral vectors are useful for gene transfer because they have a large cloning capacity and a broad tropism. Although procedures for lentiviral vector production have been standardized, simple methods to create higher titer virus during production would have extensive and important applications for both research and clinical use. Here we present a simple and inexpensive method to increase the titer by 3- to 8-fold for both integration-competent lentivirus and integration-deficient lentivirus. This is achieved during standard lentiviral production by the addition of caffeine to a final concentration of 2-4mM. We find that sodium butyrate, a histone deacetylase inhibitor shown previously to increase viral titer, works only ∼50% as well as caffeine. We also show that the DNA-PKcs (DNA-dependent protein kinase catalytic subunit) inhibitor NU7026 can also increase viral titer, but that the combination of caffeine and NU7026 is not more effective than caffeine alone. We show that the time course of caffeine treatment is important in achieving a higher titer virus, and is most effective when caffeine is present from 17 to 41 hr posttransfection. Last, although caffeine increases lentiviral vector titer, it has the opposite effect on the titer of adeno-associated virus type 2 vector. Together, these results provide a novel, simple, and inexpensive way to significantly increase the titer of lentiviral vectors.

Original languageEnglish (US)
Pages (from-to)93-100
Number of pages8
JournalHuman Gene Therapy
Volume22
Issue number1
DOIs
StatePublished - Jan 1 2011

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Lentivirus
Caffeine
Viral Load
DNA-Activated Protein Kinase
Genes
Dependovirus
Virology
Histone Deacetylase Inhibitors
Tropism
Butyric Acid
Genetic Therapy
Organism Cloning
Catalytic Domain
Clinical Trials
Research

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Ellis, B. L., Potts, P. R., & Porteus, M. H. (2011). Creating higher titer lentivirus with caffeine. Human Gene Therapy, 22(1), 93-100. https://doi.org/10.1089/hum.2010.068

Creating higher titer lentivirus with caffeine. / Ellis, Brian L.; Potts, Patrick Ryan; Porteus, Matthew H.

In: Human Gene Therapy, Vol. 22, No. 1, 01.01.2011, p. 93-100.

Research output: Contribution to journalArticle

Ellis, BL, Potts, PR & Porteus, MH 2011, 'Creating higher titer lentivirus with caffeine', Human Gene Therapy, vol. 22, no. 1, pp. 93-100. https://doi.org/10.1089/hum.2010.068
Ellis, Brian L. ; Potts, Patrick Ryan ; Porteus, Matthew H. / Creating higher titer lentivirus with caffeine. In: Human Gene Therapy. 2011 ; Vol. 22, No. 1. pp. 93-100.
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