CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Jun Wu, Marcela Vilarino, Keiichiro Suzuki, Daiji Okamura, Yanina Soledad Bogliotti, Insung Park, Joan Rowe, Bret McNabb, Pablo Juan Ross, Juan Carlos Izpisua Belmonte

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14 Scopus citations

Abstract

Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

Original languageEnglish (US)
Article number10487
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017
Externally publishedYes

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Cite this

Wu, J., Vilarino, M., Suzuki, K., Okamura, D., Bogliotti, Y. S., Park, I., ... Belmonte, J. C. I. (2017). CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs. Scientific reports, 7(1), [10487]. https://doi.org/10.1038/s41598-017-08596-5