Critical roles for Dicer in the female germline

Elizabeth P. Murchison, Paula Stein, Zhenyu Xuan, Hua Pan, Michael Q. Zhang, Richard M. Schultz, Gregory J. Hannon

Research output: Contribution to journalArticle

373 Citations (Scopus)

Abstract

Dicer is an essential component of RNA interference (RNAi) pathways, which have broad functions in gene regulation and genome organization. Probing the consequences of tissue-restricted Dicer loss in mice indicates a critical role for Dicer during meiosis in the female germline. Mouse oocytes lacking Dicer arrest in meiosis I with multiple disorganized spindles and severe chromosome congression defects. Oogenesis and early development are times of significant post-transcriptional regulation, with controlled mRNA storage, translation, and degradation. Our results suggest that Dicer is essential for turnover of a substantial subset of maternal transcripts that are normally lost during oocyte maturation. Furthermore, we find evidence that transposon-derived sequence elements may contribute to the metabolism of maternal transcripts through a Dicer-dependent pathway. Our studies identify Dicer as central to a regulatory network that controls oocyte gene expression programs and that promotes genomic integrity in a cell type notoriously susceptible to aneuploidy.

Original languageEnglish (US)
Pages (from-to)682-693
Number of pages12
JournalGenes and Development
Volume21
Issue number6
DOIs
StatePublished - Mar 15 2007
Externally publishedYes

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Oocytes
Meiosis
Mothers
Oogenesis
RNA Stability
Protein Biosynthesis
Aneuploidy
RNA Interference
Chromosomes
Genome
Gene Expression
Genes

Keywords

  • Dicer
  • Meiosis
  • Oocyte
  • RNAi

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Murchison, E. P., Stein, P., Xuan, Z., Pan, H., Zhang, M. Q., Schultz, R. M., & Hannon, G. J. (2007). Critical roles for Dicer in the female germline. Genes and Development, 21(6), 682-693. https://doi.org/10.1101/gad.1521307

Critical roles for Dicer in the female germline. / Murchison, Elizabeth P.; Stein, Paula; Xuan, Zhenyu; Pan, Hua; Zhang, Michael Q.; Schultz, Richard M.; Hannon, Gregory J.

In: Genes and Development, Vol. 21, No. 6, 15.03.2007, p. 682-693.

Research output: Contribution to journalArticle

Murchison, EP, Stein, P, Xuan, Z, Pan, H, Zhang, MQ, Schultz, RM & Hannon, GJ 2007, 'Critical roles for Dicer in the female germline', Genes and Development, vol. 21, no. 6, pp. 682-693. https://doi.org/10.1101/gad.1521307
Murchison EP, Stein P, Xuan Z, Pan H, Zhang MQ, Schultz RM et al. Critical roles for Dicer in the female germline. Genes and Development. 2007 Mar 15;21(6):682-693. https://doi.org/10.1101/gad.1521307
Murchison, Elizabeth P. ; Stein, Paula ; Xuan, Zhenyu ; Pan, Hua ; Zhang, Michael Q. ; Schultz, Richard M. ; Hannon, Gregory J. / Critical roles for Dicer in the female germline. In: Genes and Development. 2007 ; Vol. 21, No. 6. pp. 682-693.
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