Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines

Mark L. Levitt, Adi F. Gazdar, Herbert K. Oie, Hildegarde Schuller, Scott M. Thacher

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Lung carcinoma cell lines were analyzed in culture and in nude mouse xenograft for both morphological appearance and expression of specific proteins that participate in cross-linked envelope formation during normal squamous cell terminal differentiation. Cross-linked envelope formation, induced by artificial influx of millimolar Ca2+ into the cultured cells, was an exclusive trait of squamous, adenosquamous, and mucoepidermoid carcinomas. Small cell lung carcinoma and nonsquamous non-small cell lung carcinoma lines, such as adenocarcinoma and large cell carcinoma, were uniformly negative for cross-linked envelope formation. Involucrin, which is incorporated into the cross-linked envelope by the enzyme transglutaminase, was expressed at highest levels in squamous tumors, but several of the non-squamous non-small cell lung carcinoma lines also expressed comparable amounts. On the other hand, transglutaminase activity was consistently higher in squamous as opposed to nonsquamous lines, so that in cell culture, a clear contrast between the groups could be observed. A Mr 195,000 protein that is incorporated into cultured human epidermal cell cross-linked envelopes was also observed in some but not all of the squamous lines. Two forms of transglutaminase are expressed in cultured keratinocytes. One of them, tissue transglutaminase, was expressed in the majority of squamous cell lines even though it is not a normal product of squamous differentiation in vivo. Keratinocyte transglutaminase, which is distinct from the tissue form and is normally expressed during terminal differentiation in squamous epithelia, was measurably present in only one of the six squamous cell lines tested. In nude mouse xenografts, keratinocyte transglutaminase, localized immunohistochemically with a biotinylated mouse monoclonal antibody, was again present only in a minority of the squamous lines whereas involucrin was expressed in all. In contrast to involucrin, keratinocyte transglutaminase is not an obligatory component of squamous differentiation in the pulmonary carcinoma cell lines tested. Its expression may be of value in further refining their classification.

Original languageEnglish (US)
Pages (from-to)120-128
Number of pages9
JournalCancer Research
Volume50
Issue number1
StatePublished - Jan 1 1990

Fingerprint

Transglutaminases
Differentiation Antigens
Lung Neoplasms
Keratinocytes
Cell Line
Epithelial Cells
Heterografts
Nude Mice
Non-Small Cell Lung Carcinoma
Adenosquamous Carcinoma
Mucoepidermoid Carcinoma
Carcinoma
Large Cell Carcinoma
Lung
Small Cell Lung Carcinoma
Cell Differentiation
Squamous Cell Carcinoma
Cultured Cells
Proteins
Adenocarcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Levitt, M. L., Gazdar, A. F., Oie, H. K., Schuller, H., & Thacher, S. M. (1990). Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Research, 50(1), 120-128.

Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. / Levitt, Mark L.; Gazdar, Adi F.; Oie, Herbert K.; Schuller, Hildegarde; Thacher, Scott M.

In: Cancer Research, Vol. 50, No. 1, 01.01.1990, p. 120-128.

Research output: Contribution to journalArticle

Levitt, ML, Gazdar, AF, Oie, HK, Schuller, H & Thacher, SM 1990, 'Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines', Cancer Research, vol. 50, no. 1, pp. 120-128.
Levitt, Mark L. ; Gazdar, Adi F. ; Oie, Herbert K. ; Schuller, Hildegarde ; Thacher, Scott M. / Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. In: Cancer Research. 1990 ; Vol. 50, No. 1. pp. 120-128.
@article{d60dff45e86f442da9433ba7f95f61f9,
title = "Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines",
abstract = "Lung carcinoma cell lines were analyzed in culture and in nude mouse xenograft for both morphological appearance and expression of specific proteins that participate in cross-linked envelope formation during normal squamous cell terminal differentiation. Cross-linked envelope formation, induced by artificial influx of millimolar Ca2+ into the cultured cells, was an exclusive trait of squamous, adenosquamous, and mucoepidermoid carcinomas. Small cell lung carcinoma and nonsquamous non-small cell lung carcinoma lines, such as adenocarcinoma and large cell carcinoma, were uniformly negative for cross-linked envelope formation. Involucrin, which is incorporated into the cross-linked envelope by the enzyme transglutaminase, was expressed at highest levels in squamous tumors, but several of the non-squamous non-small cell lung carcinoma lines also expressed comparable amounts. On the other hand, transglutaminase activity was consistently higher in squamous as opposed to nonsquamous lines, so that in cell culture, a clear contrast between the groups could be observed. A Mr 195,000 protein that is incorporated into cultured human epidermal cell cross-linked envelopes was also observed in some but not all of the squamous lines. Two forms of transglutaminase are expressed in cultured keratinocytes. One of them, tissue transglutaminase, was expressed in the majority of squamous cell lines even though it is not a normal product of squamous differentiation in vivo. Keratinocyte transglutaminase, which is distinct from the tissue form and is normally expressed during terminal differentiation in squamous epithelia, was measurably present in only one of the six squamous cell lines tested. In nude mouse xenografts, keratinocyte transglutaminase, localized immunohistochemically with a biotinylated mouse monoclonal antibody, was again present only in a minority of the squamous lines whereas involucrin was expressed in all. In contrast to involucrin, keratinocyte transglutaminase is not an obligatory component of squamous differentiation in the pulmonary carcinoma cell lines tested. Its expression may be of value in further refining their classification.",
author = "Levitt, {Mark L.} and Gazdar, {Adi F.} and Oie, {Herbert K.} and Hildegarde Schuller and Thacher, {Scott M.}",
year = "1990",
month = "1",
day = "1",
language = "English (US)",
volume = "50",
pages = "120--128",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "1",

}

TY - JOUR

T1 - Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines

AU - Levitt, Mark L.

AU - Gazdar, Adi F.

AU - Oie, Herbert K.

AU - Schuller, Hildegarde

AU - Thacher, Scott M.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Lung carcinoma cell lines were analyzed in culture and in nude mouse xenograft for both morphological appearance and expression of specific proteins that participate in cross-linked envelope formation during normal squamous cell terminal differentiation. Cross-linked envelope formation, induced by artificial influx of millimolar Ca2+ into the cultured cells, was an exclusive trait of squamous, adenosquamous, and mucoepidermoid carcinomas. Small cell lung carcinoma and nonsquamous non-small cell lung carcinoma lines, such as adenocarcinoma and large cell carcinoma, were uniformly negative for cross-linked envelope formation. Involucrin, which is incorporated into the cross-linked envelope by the enzyme transglutaminase, was expressed at highest levels in squamous tumors, but several of the non-squamous non-small cell lung carcinoma lines also expressed comparable amounts. On the other hand, transglutaminase activity was consistently higher in squamous as opposed to nonsquamous lines, so that in cell culture, a clear contrast between the groups could be observed. A Mr 195,000 protein that is incorporated into cultured human epidermal cell cross-linked envelopes was also observed in some but not all of the squamous lines. Two forms of transglutaminase are expressed in cultured keratinocytes. One of them, tissue transglutaminase, was expressed in the majority of squamous cell lines even though it is not a normal product of squamous differentiation in vivo. Keratinocyte transglutaminase, which is distinct from the tissue form and is normally expressed during terminal differentiation in squamous epithelia, was measurably present in only one of the six squamous cell lines tested. In nude mouse xenografts, keratinocyte transglutaminase, localized immunohistochemically with a biotinylated mouse monoclonal antibody, was again present only in a minority of the squamous lines whereas involucrin was expressed in all. In contrast to involucrin, keratinocyte transglutaminase is not an obligatory component of squamous differentiation in the pulmonary carcinoma cell lines tested. Its expression may be of value in further refining their classification.

AB - Lung carcinoma cell lines were analyzed in culture and in nude mouse xenograft for both morphological appearance and expression of specific proteins that participate in cross-linked envelope formation during normal squamous cell terminal differentiation. Cross-linked envelope formation, induced by artificial influx of millimolar Ca2+ into the cultured cells, was an exclusive trait of squamous, adenosquamous, and mucoepidermoid carcinomas. Small cell lung carcinoma and nonsquamous non-small cell lung carcinoma lines, such as adenocarcinoma and large cell carcinoma, were uniformly negative for cross-linked envelope formation. Involucrin, which is incorporated into the cross-linked envelope by the enzyme transglutaminase, was expressed at highest levels in squamous tumors, but several of the non-squamous non-small cell lung carcinoma lines also expressed comparable amounts. On the other hand, transglutaminase activity was consistently higher in squamous as opposed to nonsquamous lines, so that in cell culture, a clear contrast between the groups could be observed. A Mr 195,000 protein that is incorporated into cultured human epidermal cell cross-linked envelopes was also observed in some but not all of the squamous lines. Two forms of transglutaminase are expressed in cultured keratinocytes. One of them, tissue transglutaminase, was expressed in the majority of squamous cell lines even though it is not a normal product of squamous differentiation in vivo. Keratinocyte transglutaminase, which is distinct from the tissue form and is normally expressed during terminal differentiation in squamous epithelia, was measurably present in only one of the six squamous cell lines tested. In nude mouse xenografts, keratinocyte transglutaminase, localized immunohistochemically with a biotinylated mouse monoclonal antibody, was again present only in a minority of the squamous lines whereas involucrin was expressed in all. In contrast to involucrin, keratinocyte transglutaminase is not an obligatory component of squamous differentiation in the pulmonary carcinoma cell lines tested. Its expression may be of value in further refining their classification.

UR - http://www.scopus.com/inward/record.url?scp=0025095532&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025095532&partnerID=8YFLogxK

M3 - Article

VL - 50

SP - 120

EP - 128

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 1

ER -